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酶响应光标签策略用于肠道细菌的体内成像

Wenchao Lang¹, Dunji Shu², Songhan Liu¹

¹School of Chemistry, Chemical Engineering & Biotechnology, Nanyang Technological University, Singapore 637371.

The Journal of organic chemistry

|April 12, 2024

概括

研究人员开发了新的分子探针,专门识别和图像肠道细菌表达myrosinase (Myr). 这一突破使得在活体系统中可视化细菌Myr活动,有助于健康和疾病研究.

科学领域:

生物化学生物化学
微生物学微生物学
化学生物学化学生物学

背景情况:

氨酶 (Myr) 是葡萄糖酸盐代谢中的关键酶,对肠道平衡和人类健康至关重要.
由于缺乏特定的方法,检测和可视化活体系统中的细菌Myr活动具有挑战性.

研究的目的:

开发和验证新的分子探测器,用于特异性识别和光成像表达myrosinase的肠道细菌.
为研究细菌Myr活性提供可靠的 in vitro 和 in vivo 工具.

主要方法:

合成一个人工的葡萄糖酸盐探针与一个酸盐组.
点击探头与光部分的结合.
细菌菌酶酶的酶分裂产生反应性异硫酸酸盐用于共价标签.
在活细菌菌株和动物模型中对标记细菌进行光成像.

主要成果:

开发的探针专门识别和标记表达米洛西纳酶的肠道细菌.
通过各种波长通道实现了细菌Myr活动的局部光成像.
该方法在体外细菌培养和体内动物模型中都表现出有效性.

结论:

已经建立了一种新的选择性方法来标记和成像细菌髓酶活性.
这项技术为评估化疗预防,研究肠道菌群代谢和探索肠道细菌介导的病理生理学提供了有前途的工具.

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