自然废物对体外氧化DNA损伤的影响
Dua Danjolli-Hashani1, Sebnem Selen Isbilir2
1Department of Chemistry, Institute of Natural and Applied Sciences, Trakya University, Edirne, Türkiye.
Natural product research
|April 12, 2024
概括
来自Cotinus coggygria叶和Punica granatum皮的天然提取物可以保护DNA免受氧化损伤. 这些植物废弃物中的生物活性化合物显示出用于化品和药物应用的潜力.
科学领域:
- 自然产品化学 自然产品化学
- 氧化压力研究研究 氧化压力研究
- 对DNA损伤的研究研究.
背景情况:
- 氧化性DNA损伤是衰老和疾病的一个重要因素.
- 自然废物产品是生物活性化合物的潜在来源.
- 芬顿反应是一种常见的方法,用于在体外诱导氧化应激.
研究的目的:
- 评估Cotinus coggygria叶和Punica granatum树皮提取物对氧化DNA损伤的保护作用.
- 确定负责保护作用的主要生物活性化合物.
- 探索这些天然提取物的潜在应用.
主要方法:
- 从C. coggygria树叶和P. granatum树皮中提取生物活性化合物.
- 使用芬顿反应诱导氧化DNA损伤.
- 作为DNA损伤标志物的8-基-2'-脱氧氨酸 (8-OH-2'dG) 的量化.
- 使用液体染色学-质谱学/质谱学 (LC-MS/MS) 分析生物活性化合物.
主要成果:
- 来自C. coggygria叶和P. granatum皮的乙醇提取物对DNA表现出显著的保护作用.
- 确定的主要生物活性化合物包括酸,protocatechuic 酸,myricetin,注射酸,乙基酸,ellagic 酸,abscisic 酸和phlorizin.
- 这些已识别的化合物很可能是观察到的DNA保护作用的原因.
结论:
- 科提努斯·科吉格里亚 (Cotinus coggygria) 的叶子和普尼卡·格拉纳图姆 (Punica granatum L.) 的皮肤中含有可以防止氧化DNA损伤的化合物.
- 这些自然废弃物对制药和化品行业的发展充满希望.
- 对这些提取物的具体机制和应用的进一步研究是有必要的.
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