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肠道微生物组介导的单细胞促进肝脏转移

Wenzhong Zhang¹, Jie Ling¹, Baiying Xu¹

¹Department of General Surgery, Shanghai Pudong New Area People's Hospital, Shanghai 201200, China.

International immunopharmacology

|April 12, 2024

概括

来自肝转移患者的便微生物移植通过CCL2/CCR2轴通过增加单细胞促进转移. 针对这种途径和单细胞活性可以抑制肝转移的进展.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
微生物学微生物学

背景情况:

肠道微生物群影响瘤的进展和免疫反应.
肠道微生物组调节的单细胞在肝转移中的特定作用尚未完全理解.

研究的目的:

为了研究肠道微生物组衍生的单细胞在肝脏转移中的作用.
阐明肠道微生物组促进肝脏转移的机制.

主要方法:

从肝转移 (LM) 患者和健康捐赠者 (HD) 的便微生物组移植 (FMT) 进入小鼠模型.
通过CCL2/CCR2轴对单细胞上调的分析.
抑制CCL2/CCR2和单细胞枯竭的实验.
血脂聚糖 (LPS) 和LPS/TLR4信号通路的评估.

主要成果:

与HD相比,LM患者的FMT显著促进了肝转移.
在LM FMT模型中,单细胞水平通过CCL2/CCR2轴在肝脏组织中得到上调.
CCL2/CCR2抑制和单细胞枯竭明显抑制了肝转移.
患者的FMT增加了血LPS度,突出显示了LPS/TLR4通路的重要性.

结论:

在肝转移患者的肠道微生物组变化促进转移.
单细胞受CCL2/CCR2轴调节,受LPS/TLR4信号的影响,是肠道微生物驱动性肝转移的关键媒介.

关键词:

在CCL2/CCR2中使用.我们的肠道微生物组.在LPS/TLR4中使用.肝脏转移的发生.单细胞 (Monocytes) 是一种单细胞.

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