通过QuEChERS提取和LC-MS/MS的方法对口腔液中精神活性药物的目标分析
Ettore Ferrari Júnior1, Victor Souza Bitencourt2, Ágatha Beatriz Mariano de Souza3
1Forensic Analysis Laboratory, Criminalistics Institute, The Civil Police of the Federal District, Brasília, Federal District, Brazil.
Journal of pharmaceutical and biomedical analysis
|April 12, 2024
概括
一种新方法准确地检测到电子舞蹈音乐 (EDM) 观众口服液中的51种精神活性物质. 药物使用经常被低估,许多人不知道他们消费的物质.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
背景情况:
- 电子舞蹈音乐 (EDM) 活动与使用各种精神活性物质有关.
- 在与会者中准确检测这些物质对于公共健康和安全至关重要.
- 口服液体分析为传统药物检测方法提供了一个非侵入性的替代方案.
研究的目的:
- 验证一种经过修改的QuEChERS方法与液体染色学-并联质谱学 (LC-MS/MS) 结合,用于检测口腔液体中的51种精神活性物质.
- 为了选额外的22种精神活性物质.
- 评估口服液样本储存条件的可靠性.
主要方法:
- 采用了经过修改的QuEChERS (快速,轻松,廉价,坚固和安全) 提取方法.
- 液体染色学-双重质谱法 (LC-MS/MS) 用于敏感和特定的检测.
- 采集了口服液体样本,并在-20°C下储存,并评估了储存的有效性.
主要成果:
- 经过验证的方法表现出极好的性能,检测极限低 (0.040.5 ng/mL) 和量化极限低 (0.11.5 ng/mL).
- 3,4-甲基二氧化甲胺 (MDMA),甲基二氧化甲胺 (MDA),可卡因及其代谢物和甲胺经常被检测到.
- 分析显示,在EDM参与者中,非法物质使用的报告数量显著不足,许多人甚至不知道自己的使用情况.
结论:
- 开发的LC-MS/MS方法对口服液中广泛的精神活性物质的同时确定和查是有效的.
- 口服液是适合在活动环境中进行药物测试的矩阵,储存条件可靠.
- 调查结果强调了EDM参与者自我报告和实际使用药物之间的关键差距,强调了针对性干预和教育的必要性.
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