生物工艺优化,以一种新型固态发酵生物反应器从麦废料中生产食品级的纤维溶解酶,用于增强果汁澄清
Raikamal Bhattacharya1, Sidharth Arora2, Sanjoy Ghosh1
1Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India.
Journal of environmental management
|April 12, 2024
概括
这项研究表明,使用Trichoderma reesei在一个包装床生物反应器中,有效地从农业废物中产生食品级细胞酶. 这些酶提高了果汁的质量,并提供可持续的生物炼油解决方案.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物精炼是生物精炼的一种方式.
背景情况:
- 农业废物是一个可持续性挑战.
- 废物的生物转化成有价值的产品,如酶,符合联合国可持续发展目标.
- 工业酶对于各种应用,包括食品加工至关重要.
研究的目的:
- 探索使用农业废物从Trichoderma reesei中生产高产,食品级的纤维溶解酶.
- 通过介质配方和过程参数控制来优化酶生产.
- 评估产生的酶在食品应用中的有效性和残留物用于燃料颗粒的潜力.
主要方法:
- 使用的废石榴草和棉油蛋糕作为Trichoderma reesei MTCC 4876在固态发酵 (SSF) 的基质.
- 在一个带有控制通风的6L包装床生物反应器 (PBR) 中,优化了介质组件和物理参数.
- 使用微过和超过净化纤维化酶缩物,并评估其稳定性和安全性.
主要成果:
- 在高真菌生物量下,实现了高细胞酶 (20.64 FPU/g-ds) 和西兰酶 (16,186 IU/g-ds) 的高产量.
- 与摇动瓶相比,PBR系统显示出更高的生产率.
- 食品级细胞质缩剂显著改善了果汁的清晰度,降低了杂度和粘度,增加了降解糖.
- 发酵残留物显示出可持续燃料颗粒的潜力.
结论:
- 开发了一种高效,可扩展的工艺,用于在PBR中使用SSF从农业废物中生产食品级细胞质.
- 纯化的细胞质缩物是稳定的,安全的食品应用,并提高水果汁的质量.
- 该研究强调了可持续的生物炼油方法,将废物转化为有价值的酶和潜在的燃料来源.
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