来自人类多能干细胞的寄生神经元模拟人类疾病和发育
Hsueh-Fu Wu1, Kenyi Saito-Diaz2, Chia-Wei Huang3
1Center for Molecular Medicine, University of Georgia, Athens, GA 30602, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
Cell stem cell
|April 12, 2024
概括
人类多能干细胞衍生的寄同情神经元为研究自主神经系统发育和家族性脱自主症和Sjögren综合征等疾病提供了一种新型模型.
科学领域:
- 神经科学是一个神经科学.
- 干细胞生物学 干细胞生物学
- 发展生物学 发展生物学
背景情况:
- 自主性副交感神经元 (parasymNs) 调节重要的"休息和消化"功能,对器官发育至关重要.
- 帕拉西姆尼功能障碍与自主神经病变有关,但由于缺乏合适的模型系统,人类研究受到限制.
- 人类多能干细胞 (hPSCs) 提供了一个多功能平台,用于产生特定的神经元亚型用于研究.
研究的目的:
- 开发一种差异化方法,从 Schwann 细胞原始体中衍生出功能性人类寄生体.
- 利用这些hPSC衍生的寄生体来建模人类自主神经系统 (ANS) 发育和各种神经病变.
- 调查寄生素在疾病和发育中的作用,包括遗传性疾病,病毒感染,自身免疫性疾病和脂肪细胞成熟.
主要方法:
- 建立了一个差异化范式,从hPSC衍生的施万细胞原始体中衍生出人类寄生体.
- 利用衍生出的parasymNs来建模家族性脱机症 (FD),Sjögren综合征 (SS) 和SARS-CoV-2感染效应.
- 研究了parasymN内化及其在白色脂肪细胞 (WAT) 发育和成熟中的作用.
主要成果:
- 通过使用一种新的分化策略,成功地从施万细胞原始体中获得了功能性人类寄生体.
- 证明了这种模型系统对研究人类ANS发育和建模FD,SS和SARS-CoV-2-诱导的寄生体MN功能障碍的实用性.
- 显示了寄生素激活WAT并促进其成熟,突出显示了一种新的发育相互作用.
结论:
- 已经建立了一个使用hPSC衍生的寄生体的新模型系统来研究人类自主神经系统的发展.
- 这种模型使得可以在各种人类疾病中调查parasymN功能障碍,包括遗传,自身免疫和传染病.
- 开发的平台对未来的疾病建模,药物发现和推进我们对人类神经发育的理解有很大的潜力.
关键词:
在 COVID-19 疫情中,施万细胞的原始细胞斯约格伦综合征是什么意思脂肪细胞内置的内置.自主神经系统自主神经系统疾病建模 疾病建模一个家庭的自主权.人类多能干细胞干细胞寄同情神经元是一种神经元.周围神经系统 周围神经系统更多相关视频
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