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Updated: Jun 28, 2025

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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由菌体显示器选择的反平行α螺旋和β片组成的蛋白质-蛋白质结合表面的实用性

Ningyu Zhu1, Philip M Smallwood1, Amir Rattner1

  • 1Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, USA; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, USA.

The Journal of biological chemistry
|April 12, 2024
PubMed
概括

研究人员探索了新的蛋白质支架,CC4和FN3域,用于设计多功能蛋白质结合试剂. 这些新型支架显示出在细胞成像和向传递系统中的应用潜力.

关键词:
在Fc融合蛋白质中.雷克 (RECK) 的意思是说.亲和关系是一种亲和关系.纤维肌肉生殖素的使用.菌体显示器可以显示菌体.蛋白质工程工程 蛋白质工程蛋白质的进化 蛋白质的进化蛋白质与蛋白质的相互作用

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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