系统识别和表征外体内环RNAs的系统识别和表征
Yinchun Zhong1, Yan Yang2, Xiaolin Wang2
1Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China.
Genome research
|April 12, 2024
概括
我们开发了FEICP来识别外体内环RNAs (EIciRNAs),并发现它们与其他环RNAs不同. SRSF1蛋白调节EIciRNA的产生,影响神经元的分化.
科学领域:
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
背景情况:
- 由于缺乏生物信息学工具,保留了内子的外体内环RNA (EIciRNAs) 尚未得到充分研究.
- 了解EIciRNA调节和功能对于推进RNA生物学至关重要.
- 在EIciRNAs中的内部保留 (IR) 提出了独特的研究挑战.
研究的目的:
- 从高吞吐量测序 (HTS) 数据中开发一个有效的EIciRNA检测的计算框架.
- 描述EIciRNAs及其相关的保留内子 (CIRs) 的全球特征.
- 研究EIciRNAs的调节机制和生理功能,特别是在神经元分化中.
主要方法:
- 开发FEICP (Find Exon-Intron circRNAs) 管道,用于EIciRNA的识别.
- 应用深度学习模型来分析CIR与线性内子 (LIR) 相比的序列特征.
- 全基因组CRISPR查和计算分析以确定调控因素,包括SRSF1.1.
主要成果:
- FEICP成功检测到EIciRNAs,揭示了不同的特征,包括更大的尺寸,核定位,组织特异性和大脑丰富.
- CIR比LIR更短,具有较弱的拼接部位,具有更高的GC含量,形成更多的二次结构,并且比LIR更保守.
- SRSF1被确定为一种关键的调节器,可以结合CIR并抑制EIciRNA生物发生,在调节EIciLIMK1神经元分化方面发挥着特定的作用.
结论:
- FEICP管道提供了一种有效的方法,用于从HTS数据中识别EIciRNA.
- EIciRNAs表现出独特的分子特征,并受到SRSF1.1.等因素的调节.
- EIciRNAs在细胞过程中起着重要的作用,以它们参与神经元分化为例.
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