在传统的大豆 (meju) 发酵过程中,单糖,寡糖和微生物社区特征变化之间的相关性
HyunJi Lee1, Eunhye Jo2, JaeHui Song1
1Department of Applied Chemistry ⋅ Food Science and Technology, Dong-eui University, Busan 47340, Republic of Korea.
Food research international (Ottawa, Ont.)
|April 12, 2024
概括
这项研究确定了韩国发酵大豆食品 (meju) 中的关键微生物,并揭示了它们如何转化碳水化合物. 它强调了真菌 (如Mucor) 和细菌 (如Weissella) 在发酵过程中分解和利用糖的作用.
科学领域:
- 食品微生物学 食品微生物学
- 发酵科学 发酵科学
- 生物化学 生化学
背景情况:
- 梅朱是韩国美食中重要的发酵大豆初始培养物.
- 了解meju发酵期间的碳水化合物转化对于传统食品质量至关重要.
研究的目的:
- 在自发发酵的果汁中识别本土微生物.
- 在发酵过程中表征单糖和寡糖的变化.
- 阐明特定微生物在碳水化合物降解和利用中的作用.
主要方法:
- 使用metabarcoding进行微生物社区分析.
- 通过高性能液态染色学-四极点飞行时间质谱法 (HPLC-Q-TOF MS) 进行单糖样化.
- 使用高性能液体染色学-质谱学/质谱学 (HPLC-MS/MS) 进行寡糖的分析.
主要成果:
- 主导细菌包括韦塞拉,乳酸菌和Leuconostoc;粘菌是最丰富的真菌.
- 在发酵过程中,单糖的总含量显著增加.
- 寡糖化物分析显示大豆食纤维降解,并揭示了新的结构,Mucor与糖寡糖化物降解和利用糖化物的细菌有关.
结论:
- 这项研究确定了参与中碳水化合物代谢的关键微生物参与者 (Mucor,Weissella).
- 粘液有助于复杂的大豆多糖的降解.
- 像韦塞拉 (Weissella) 这样的细菌属似乎利用释放的糖,驱动发酵过程.
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