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评估人类神经毒性和毒理相互作用的影响,同时发生的调节和新兴的真菌毒素

Soraia V M de Sá¹, Carolina Sousa Monteiro¹, José O Fernandes¹

¹LAQV-REQUIMTE, Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.

Food research international (Ottawa, Ont.)

|April 12, 2024

概括

这项研究评估了常见食品菌毒素在人类神经细胞中的综合神经毒性. 奥克拉托克素A和斯特里格马托西斯的组合显示出显著的协同效应,突出了食品中同时出现的真菌毒素带来的风险.

科学领域:

毒理学毒理学毒理学
神经科学是一个神经科学.
食品安全食品安全

背景情况:

菌毒素在食品中普遍存在,并与神经退行性疾病有关.
评估结合菌根毒素的毒性对于理解人类健康风险至关重要.
以谷物为基础的产品是饮食中菌毒素暴露的常见来源.

研究的目的:

在人类SH-SY5Y神经细胞中评估单个和组合的真菌毒素 (ENNB,STG,AFB1,CPZ,CIT,OTA) 的细胞毒性.
为了确定菌毒素组合的相互作用类型 (协同作用,添加剂,对抗作用).
为了确定具有显著协同作用或对抗作用的特定真菌毒素对.

主要方法:

使用中位数效应方程 (Chou 和 Talalay 的方法) 的细胞毒性评估.
测试单个真菌毒素及其二元和二元组合.
通过人类SH-SY5Y神经细胞的IC50值来量化神经毒性.

主要成果:

恩雅丁B (ENNB) 是最具神经毒性的个人菌毒素,其次是Ochratoxin A (OTA) 和Sterigmatocystin (STG).
OTA + STG组合显示了最高的联合毒性,而CPZ + CIT则是最低的.
大约70%的二元组合表现出协同效应或添加效应;OTA主要表现出协同效应 (90%),而ENNB倾向于对抗.

结论:

菌毒素在食品中的同时存在可能导致显著的协同神经毒性作用,增加人类暴露的危险.
STG + CIT组合显示出了显著的协同作用,降低了相应毒性所需的剂量.
准确评估结合性真菌毒素暴露对公共健康至关重要,特别是考虑到它们在食物和环境中经常存在.

关键词:

谷物谷物谷物组合指数是指组合指数.细胞毒性细胞毒性人类暴露的人类暴露.相互作用相互作用在SH-SY5Y细胞中.

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