Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Triple Functions of D2 Silencing in Treatment of Periapical Disease.

Journal of endodontics·2017
Same author

Excellent Thermoelectric Properties in monolayer WSe<sub>2</sub> Nanoribbons due to Ultralow Phonon Thermal Conductivity.

Scientific reports·2017
Same author

Activation of Akt by SC79 protects myocardiocytes from oxygen and glucose deprivation (OGD)/re-oxygenation.

Oncotarget·2017
Same author

ColorSketch: A Drawing Assistant for Generating Color Sketches from Photos.

IEEE computer graphics and applications·2017
Same author

Enclosure Transform for Interest Point Detection From Speckle Imagery.

IEEE transactions on medical imaging·2017
Same author

Altered brain structural networks in attention deficit/hyperactivity disorder children revealed by cortical thickness.

Oncotarget·2017

相关实验视频

Updated: Jun 28, 2025

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
11:59

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

7.3K

在血小板捐献者中对CD36的多态性分析.

Qilu Lyu1,2, Yuwei Lin1,2,3, Yiming Pan1,2

  • 1Clinical Transfusion Research Center, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, China.

Scientific reports
|April 12, 2024
PubMed
概括

CD36缺乏症涉及改变的CD36基因和蛋白质水平. 这项研究确定了流行突变,并将RNA变异与II型缺陷联系起来,表明可溶性CD36 (sCD36) 是一个潜在的生物标志物.

关键词:
CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36 CD36血小板捐赠者 血小板捐赠者多态性多态性多态性sCD3636 在线观看

更多相关视频

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting
07:27

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

Published on: May 16, 2013

18.8K
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

11.8K

相关实验视频

Last Updated: Jun 28, 2025

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
11:59

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

7.3K
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting
07:27

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

Published on: May 16, 2013

18.8K
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

11.8K

科学领域:

  • 遗传学和分子生物学
  • 免疫学 免疫学 免疫学
  • 生物化学 生物化学

背景情况:

  • CD36缺乏症的特征是CD36在血小板和/或单细胞上的缺陷,目前还不完全理解分子和蛋白质表达水平之间的相关性.
  • 研究这些相关性对于理解CD36缺乏症的病理生理学至关重要.

研究的目的:

  • 确定CD36基因的多态性,其RNA水平,以及在血小板和血中的CD36蛋白表达.
  • 为了将遗传变异与CD36缺乏的分子和蛋白质表达变化相关联.

主要方法:

  • 桑格对CD36基因多态性的测序.
  • 使用HotMuSiC,CUPSAT,SAAFEC-SEQ和FoldX进行生物信息分析.
  • 定量PCR (qPCR),流细胞计和ELISA用于RNA和蛋白质水平分析.

主要成果:

  • 确定了c.1228_1239delATTGTGCCTATT作为最常见的突变 (等位基因频率为0.0072).
  • 在细胞外域确认了5种突变,其中4种 (c.284T>C,c.512A>G,c.572C>T,c.869T>C) 预计会对CD36蛋白稳定性产生有害影响.
  • 在突变载体和缺陷个体中观察到血小板CD36表达的显著较低的平均光强度 (MFI).
  • 与对照人群相比,在II型缺乏症中发现可溶性CD36 (sCD36) 水平显著降低,在II型血小板患者中发现较低的CD36RNA水平.

结论:

  • RNA水平的变化可能是II型CD36缺乏症的基础.
  • 在昆明队列中发现了流行突变c.1228_1239delATTGTGCCTATT.
  • 可溶性CD36 (sCD36) 显示出作为评估CD36缺乏个体免疫反应风险的生物标志物的潜力,需要进一步调查.