开发尿路生物标志物的侧流装置,以评估脏健康状况

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Serena D Souza¹, Wassim Obeid¹, Jeanine Hernandez¹

¹Division of Nephrology, Department of Medicine, Johns Hopkins University School of Medicine, 1830 E. Monument St., Suite 416, Baltimore, MD, 21287, USA.

Scientific reports

|April 12, 2024

概括

这项研究开发了新的横流器 (LFD) 来检测尿道泌尿素 (UMOD) 和骨质 (OPN),为评估脏健康提供了快速的护理点方法. 在人类尿样中,LFDs成功量化了UMOD和OPN.

科学领域:

生物标志物的发现和开发.
护理点诊断的诊断方法
评估功能评估功能评估

背景情况:

血清肌素是实时功能变化的不敏感标记物.
尿路生物标志物如尿素 (UMOD),骨质素 (OPN) 和中白素-9 (IL-9) 显示出对脏健康评估的前景.
需要快速的护理点测试来改善生物标志物的可访问性.

研究的目的:

开发新型侧流装置 (LFD),用于同时检测尿道UMOD,OPN和IL-9.
建立一个快速和可访问的诊断工具,用于监测损伤和健康.
验证开发的LFD用于UMOD和OPN检测的性能.

主要方法:

使用配对抗体开发侧向流装置 (LFD).
酶相关免疫吸收试验 (ELISA) 用于抗体查.
优化结合缓冲器pH值和检测抗体度.
量化测量UMOD和OPN标准和人类尿液样本.

主要成果:

对UMOD和OPN成功开发了LFD,但IL-9没有.
对于LFD发展的最佳条件包括7.8或8.5的结合缓冲 pH 和15μg/mL检测抗体度.
在LFD中,UMOD的低检测极限为80,000 pg/mL,OPN的低检测极限为8,600 pg/mL.
开发的LFD准确地测量了人体尿中的UMOD和OPN,其百分比变化系数 (CV) 分别为12.12%和5.23%.

结论:

针对尿道UMOD和OPN的新型LFD已经成功开发出来.
这些LFD提供了一种定量和快速的方法来评估脏健康.
开发的临床检测具有早期发现和治疗损伤的潜力.

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