凯姆菲罗尔通过通过PI3K/AKT和MAPK信号通路调节葡萄糖摄入量,线粒体生物发生和蛋白质合成来提高运动性能
Xiaoning Ji1,2, Chaozheng Zhang2, Jing Yang2
1State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Foods (Basel, Switzerland)
|April 13, 2024
概括
凯姆菲罗尔是一种天然的黄类化合物,可以提高运动表现,并减少小鼠的疲劳. 它增强抗氧化能力,能量储存 (糖原,ATP) 和葡萄糖水平,同时改善细胞培养中的肌肉功能.
科学领域:
- 营养生物化学 营养生物化学
- 运动生理学 运动生理学
- 分子生物学分子生物学
背景情况:
- 凯姆费醇是一种广泛存在的天然黄类化合物,具有多种生物活性.
- 它对运动表现和肌肉新陈代谢的具体影响需要进一步阐明.
- 了解kaempferol的机制可以揭示性能提升的新策略.
研究的目的:
- 为了在体内研究凯姆菲罗尔对运动表现的影响.
- 在实验室中探索kaempferol在肌肉细胞中的潜在分子机制.
- 评估kaempferol对疲劳标记,抗氧化能力和能量代谢的影响.
主要方法:
- 使用小鼠进行体内研究,以测量握力,跑步时间和距离.
- 对疲劳标记,抗氧化酶 (SOD,GSH-Px),糖原,ATP和血糖的生物化学分析.
- 使用C2C12神经管 (2D和3D培养) 的体外研究来评估葡萄糖吸收,蛋白质合成,线粒体功能和氧化应激.
- 对PI3K/AKT和MAPK信号通路激活和基因表达的分析.
主要成果:
- 高剂量的kaempferol显著增加了小鼠的握力,跑步时间和距离.
- 凯姆菲罗尔可以减少疲劳标记,同时增强抗氧化酶活动和能量储存 (糖原,ATP,血糖).
- 在体外,kaempferol改善了葡萄糖吸收,蛋白质合成和线粒体功能,并减少了髓管中的氧化应激.
- 凯姆菲罗尔激活PI3K/AKT和MAPK通路,升调参与葡萄糖吸收,线粒体生物发生和蛋白质合成的关键基因.
结论:
- 凯姆菲罗尔有效地提高了运动表现,并减轻了身体疲劳.
- 机制包括增强的葡萄糖吸收,线粒体生物发生和蛋白质合成,以及减少氧化应激.
- 凯姆菲罗尔的作用可能通过PI3K/AKT和MAPK信号通路进行介导.
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