玉米的鉴定与酒精脱酶激活活性被Caco-2细胞单层吸收
Zhe Wang1,2, Guanlong Li2, Xiaolan Liu1,2
1College of Food Engineering, Harbin University of Commerce, Harbin 150076, China.
Molecules (Basel, Switzerland)
|April 13, 2024
概括
发现玉米蛋白质可以激活酒精脱酶 (ADH),增强酒精代谢. 这些在消化和吸收后被识别出来,显示出作为肝脏保护的功能成分的潜力.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 药理学 药理学是指药理学的学科.
背景情况:
- 酒精脱酶 (ADH) 对于乙醇代谢至关重要.
- 在体外对ADH激活活性的评估预测了体内乙醇代谢能力.
- 玉米蛋白粉 (CGM) 是生物活性的潜在来源.
研究的目的:
- 准备和识别来自CGM的ADH激活.
- 在模拟消化和吸收后评估这些的ADH激活能力.
- 探索这些的ADH激活机制.
主要方法:
- 使用酸盐的CGM的酶性水解.
- 模拟胃肠道消化过程.
- 二细胞单层模型用于的吸收.
- 的ADH激活测试.
- 分子对接模拟. 分子对接模拟.
主要成果:
- 使用酸盐的玉米蛋白质水解剂在消化后显示了最高的ADH激活 (52.93 ± 2.07%).
- 类SSNCQPF,TGCPVLQ和QPQQPW显示出具有较低EC50值的显著ADH激活.
- 分子对接证实了通过键和性相互作用形成稳定的-ADH复合体.
结论:
- 玉米蛋白质水解剂产生强大的ADH激活.
- 已识别的 (SSNCQPF,TGCPVLQ,QPQQPW) 能够有效地激活ADH.
- 玉米蛋白质水解剂显示为酒精代谢和肝脏保护的功能成分具有前景.
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