相关实验视频

Updated: Jun 28, 2025

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

从诱导的多能干干细胞衍生的脑细胞中比较细胞外囊泡

Gabriela Xavier^1,2, Alexander Navarrete Santos³, Carla Hartmann⁴

¹LiNC-Laboratory of Integrative Neuroscience, Universidade Federal de São Paulo (UNIFESP), São Paulo CEP 05039-032, Brazil.

International journal of molecular sciences

|April 13, 2024

概括

来自干细胞的外体的研究揭示了神经疾病的潜在生物标志物. 外体中的特定蛋白质模式可能有助于分类晚期发作的阿尔茨海默病,有助于未来的诊断.

科学领域:

神经科学是一个神经科学.
生物化学生物化学
干细胞生物学干细胞生物学

背景情况:

神经精神疾病缺乏明确的生物标志物,阻碍了患者的分类和治疗.
外体,循环囊泡穿越血脑屏障,携带细胞特异性分子,使它们成为有希望的生物标志物来源.
人类诱导的多能干细胞 (iPSC) 和它们的衍生物为研究脑细胞衍生的外体提供了一个模型.

研究的目的:

为了分析各种人类iPSC衍生的脑细胞类型的外体蛋白质签名.
为了识别特定于细胞类型的外体细胞表面标记物.
在晚发性阿尔茨海默病 (LOAD) 和精神分裂症 (SCZ) 中发现疾病特异性的外体特征.

主要方法:

培养的iPSC和衍生的神经干细胞,原始细胞,神经元,星球细胞,微状细胞和大脑毛细血管内皮细胞.
在特定细胞类型中分析了31种外体表面标记物以进行丰富.
与SCZ和LOAD患者健康对照 (HC) 的外体蛋白质模式进行比较.

主要成果:

在星体细胞 (CD29,CD44,CD49e),微状细胞 (CD44) 和神经干细胞 (SSEA4) 中确定了丰富的生物标志物.
与HC相比,在LOAD患者中发现了一个独特的外体表皮图模式 (CD1c,CD2).
与HC相比,SCZ没有发现显著的疾病特异性外体模式.

结论:

来自iPSC的细胞培养为研究外体蛋白质组生物标志物提供了有价值的模型.
外基因组分析显示了识别新型疾病概况的潜力,特别是对于LOAD.
对外体特征的进一步研究可能会导致神经精神疾病诊断工具的改进.

关键词:

星球细胞是星球细胞.大脑毛细血管内皮细胞 (BCECs)细胞外囊泡 (EVs) 的产生.诱导多能干细胞 (iPSCs) 是一种晚期发作的阿尔茨海默氏病 (LOAD)神经分化的神经分化.精神分裂症是一种精神分裂症.

更多相关视频

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Published on: November 8, 2024

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

相关概念视频

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...