血清诱导NF-κB在增殖的人类心脏干细胞中的子单位特异激活
Kazuko E Schmidt1,2,3, Anna L Höving1,2,3, Katja Nowak1,2,3
1Department of Cell Biology, Faculty of Biology, University of Bielefeld, 33615 Bielefeld, Germany.
International journal of molecular sciences
|April 13, 2024
概括
人类心脏干细胞的增殖对于心脏修复至关重要,被血清增强. 这项研究确定了NF-κB途径和特定的基因标,这些标参与了这种有益于心血管疾病治疗的过程.
科学领域:
- 心血管研究的心血管研究.
- 干细胞生物学 干细胞生物学
- 分子信号传递是分子信号传递.
背景情况:
- 心血管疾病 (CVD) 是全球主要的死亡原因,通常与衰老有关.
- 成人心脏干细胞的增殖减少限制了心脏的再生.
- 人类血清在增强心脏干细胞增殖和减少衰老方面表现出潜力.
研究的目的:
- 研究负责成年人心脏干细胞 (hCSCs) 血清媒介增殖的分子信号通路.
- 确定参与增强hCSC增殖潜力的关键因素,以改善基于细胞的心血管疗法.
主要方法:
- 利用NF-κB抑制剂皮罗利丁二甲酸盐 (PDTC) 探测NF-κB在hCSC增殖中的作用.
- 采用RNA测序 (RNA-Seq) 来分析对血清治疗和NF-κB抑制反应的基因表达变化.
主要成果:
- 证明了转录因子NF-κB在血清诱导的hCSC扩散中的参与.
- 确定ATF6B,COX5B和TNFRSF14作为潜在的NF-κB向基因,与血清中介的hCSC增殖有关.
结论:
- NF-κB信号传递是成人人类心脏干细胞中血清诱导的增殖的关键媒介.
- 准NF-κB及其下游基因 (ATF6B,COX5B,TNFRSF14) 可能为心血管疾病提供新的治疗策略.
更多相关视频
12:48Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
Published on: January 7, 2019
6.4K
10:46Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Published on: November 3, 2011
20.3K
相关概念视频
01:01Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
02:26NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
01:26Stem Cell Niche
5.1K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.1K