GII.P31/GII.4_悉尼_2012 诺罗病毒在日本的一家诊所十年的分子演变
Hiroshi Ushijima1, Sheikh Ariful Hoque1,2, Yuki Akari3
1Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Itabashi, Tokyo 173-8610, Japan.
International journal of molecular sciences
|April 13, 2024
概括
悉尼_2012诺基病毒 (GII.P31/GII.4_Sydney_2012) 菌株显示出显著的氨基酸变化,特别是在抗原性位点,尽管基因变化很小. 这种演变影响了病毒性腹预防策略.
科学领域:
- 病毒学 病毒学
- 分子流行病学分子流行病学
- 公共卫生 公共卫生
背景情况:
- 诺罗病毒 (NoV) 基因组II,聚合酶类型P31,体基因型4,悉尼_2012变种 (GII.P31/GII.4_Sydney_2012) 已成为十多年来流通的主要菌株.
- 了解它的进化轨迹对于有效的疾病控制至关重要.
研究的目的:
- 调查GII.P31/GII.4_Sydney_2012诺罗病毒菌株的遗传多样性和分子变化.
- 分析2012年至2022年期间来自日本的流行病菌株的所有三个开放式读取框架 (ORF) 中的氨基酸 (aa) 差异.
主要方法:
- 使用下一代测序来分析来自日本京都和静岡的14个GII.P31/GII.4_Sydney_2012菌株.
- 专注于识别体 (ORF2) 和其他ORFs的抗原区域中的氨基酸变异.
主要成果:
- 在囊体 (ORF2) 和其他ORF中的抗原位置中发现了许多重要的氨基酸变化.
- 遗传学分析显示,与后来的菌株 (2019-2022) 相比,早期菌株 (2013-2016) 在所有三个ORF中都存在明显的聚类.
结论:
- 这项研究强调了GII.P31/GII.4_Sydney_2012诺罗病毒菌株正在进行的分子进化,其特点是具有显著的aa变化而没有重大基因分歧.
- 这些发现为这种主导菌株的进化动态提供了宝贵的见解,为未来的病毒性腹预防和治疗工作提供了信息.
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