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Updated: Jun 28, 2025

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Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
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SMA20/PMIS2是一种快速进化的精子膜抗原,在受精过程中可能具有不同物种的功能
Nathaly Cormier1, Asha E Worsham2, Kinsey A Rich2
1Department of Biological Sciences, University of Wisconsin-Whitewater, Whitewater, WI 53190, USA.
International journal of molecular sciences
|April 13, 2024
概括
研究人员确定了一种新的精子蛋白质SMA20作为PMIS2,这对猪受精至关重要. 这种蛋白质是胎盘哺乳动物独有的,很可能迅速进化,在受精过程中起到特定的作用.
科学领域:
- 生殖生物学 生殖生物学
- 分子进化分子进化
- 哺乳动物遗传学 哺乳动物遗传学
背景情况:
- 精子膜的合抗原对受精至关重要.
- 在猪身上发现了一种以前未知的 Mr 20,000 精子膜特异蛋白 (SMA20).
研究的目的:
- 描述SMA20并确定其身份和进化起源.
- 为了研究SMA20在野猪精子中的表达和定位.
主要方法:
- 对SMA20的cDNA测序和分析.
- 北方涂抹以评估转录表达.
- BLASTp在其他物种中搜索同源序列.
- 免疫组织化学和超结构性免疫定位研究.
主要成果:
- 确定SMA20是PMIS2的未注释的猪正体,编码126个氨基酸蛋白质,具有两个跨膜段.
- SMA20通过丸特定的1.1 kb转录表达.
- SMA20与 warthog PMIS2 呈现高序列相同性,但在其他哺乳动物中显著分离,特别是在其 N-终端AP 丰富区域.
- 免疫活性局部化SMA20到野猪精子的体和体头部,特别是在内部的血膜,外体膜和体含量.
结论:
- 编码SMA20/PMIS2的基因在胎盘哺乳动物 (Eutheria) 中在它们从有袋动物 (Metatheria) 分离后出现了 de novo.
- SMA20/PMIS2的快速分子进化可能赋予了对胎盘哺乳动物受精至关重要的物种分离功能.
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