相关实验视频

Updated: Jun 28, 2025

Rescue of Recombinant Newcastle Disease Virus from cDNA

Rescue of Recombinant Newcastle Disease Virus from cDNA

Published on: October 11, 2013

维护了OrthohepadnavirusX蛋白质的功能,以抑制I型干扰素信号传输

Amonrat Choonnasard^1,2, Maya Shofa^1,2, Tamaki Okabayashi^1,2,3

¹Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.

International journal of molecular sciences

|April 13, 2024

概括

脊髓炎X病毒蛋白质通过降解关键适应蛋白质,保持抑制I型干扰素信号通路的能力. 这种保存的免疫逃避机制对于各种哺乳动物宿主持续感染至关重要.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

脊髓炎病毒在各种哺乳动物中引起慢性肝炎.
乙型肝炎病毒 (HBV) X蛋白抑制I型干扰素 (IFN) 信号传递,有助于病毒的持久性.
来自其他物种的Orthohepadnavirus X蛋白的IFN抑制作用在很大程度上是未知的.

研究的目的:

为了研究17个Orthohepadnavirus X蛋白质的保存的抗IFN活性.
为了确定Orthohepadnavirus X蛋白是否可以抑制IFN信号通路.

主要方法:

17个Orthohepadnavirus X蛋白的表达和分析.
评估TRIF介导的IFN-β信号通路的抑制.
对MAVS介导的IFNβ信号通路的影响的评估.

主要成果:

通过TRIF降解通过Orthohepadnavirus X蛋白质对TRIF介导的IFN-β信号通路的保留抑制.
家庭猫头病毒 (DCH) X蛋白显示出强大的抑制MAVS介导的IFNβ信号传递,类似于HBV X.
这些发现突出了Ortohepadnaviruses中免疫逃避的保存机制.

结论:

抑制I型干扰素信号传递是Orthohepadnavirus X蛋白的保留功能.
这种保存活动通过逃避宿主天生的免疫反应,有助于病毒的持久性.
这项研究揭示了Orthohepadnavirus属内的不同物种免疫逃避的共同策略.

关键词:

整形骨头上有病毒.蛋白质 X 蛋白质 X 蛋白质家庭猫肝炎病毒 (DCH)乙型肝炎病毒 (HBV)干扰子-β信号通路的信号通路.

更多相关视频

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

相关概念视频

Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

Retroviruses

Retroviruses

Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...