人体血小板中的皮质醇诱导的氧化应激.
Maria Grazia Signorello1, Silvia Ravera2, Giuliana Leoncini1
1Biochemistry Laboratory, Department of Pharmacy, University of Genoa, 16132 Genova, Italy.
International journal of molecular sciences
|April 13, 2024
概括
皮质醇通过刺激活性氧物种 (ROS) 生产和削弱抗氧化防御来显著增加人体血小板中的氧化应激. 这种荷尔蒙效应与血栓形成和心血管疾病风险有关.
科学领域:
- 生物化学 生物化学
- 血液学 血液学 血液学
- 分子生物学分子生物学
背景情况:
- 过高皮质醇症会影响血小板功能,但对外源性皮质醇的影响尚不清楚.
- 关于皮质醇对人体血小板的影响现有研究产生了相互矛盾的结果.
研究的目的:
- 为了研究外源皮质醇对人类血小板中氧化应激标志物的影响.
- 阐明参与皮质醇诱导的血小板氧化应激的酶途径和ROS来源.
主要方法:
- 分析反应性氧物种 (ROS) 生产,超氧化离子形成和脂质过氧化.
- 测量谷氨 (GSH) 和膜硫醇 (SH组) 含量.
- 评估酶参与 (src, syk, PI3K, AKT) 和使用特定途径调节剂 (例如,NADPH氧化酶抑制剂,线粒体呼吸链抑制剂) 的抑制研究.
主要成果:
- 皮质醇显著增加了ROS的产生,超氧化离子的形成,以及血小板中的脂质过氧化.
- 皮质醇降低了血小板GSH和膜SH组含量,表明抗氧化防御的耗尽.
- 证明了src, syk,PI3K和AKT酶的参与;NADPH氧化酶1活性和未合的氧化酸化被确定为关键ROS来源.
结论:
- 外源皮质醇通过增强ROS生成和降低抗氧化能力来增强人体血小板中的氧化应激.
- 皮质醇诱导的血小板氧化应激涉及NADPH氧化酶1和线粒体复合物I/III,但不涉及复合物II/IV.
- 这种皮质醇介导的血小板氧化压力可能会导致动脉和静脉血栓形成,增加心血管疾病的风险.
更多相关视频
10:00Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
Published on: December 11, 2020
2.6K
11:40Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
Published on: March 27, 2014
37.1K
相关概念视频
Hypothalamic-Pituitary Axis
60.0K
The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
60.0K
Physiological Foundation of Stress
56
Stress triggers a coordinated physiological response involving the sympathetic nervous system (SNS) and the hypothalamic-pituitary-adrenal (HPA) axis. This dual activation ensures that the body is prepared for both immediate and prolonged stress management. The process begins with the perception of a stressor. This initial phase activates the SNS, leading to the rapid release of adrenaline (epinephrine) from the adrenal glands.
Role of the Sympathetic Nervous System
Adrenaline triggers the...
Role of the Sympathetic Nervous System
Adrenaline triggers the...
56
Psychoneuroimmunology: Cardiovascular Disease
30
Psychoneuroimmunology (PNI) is a multidisciplinary field that examines how psychological factors, particularly stress, interact with the immune system and impact physical health. Research in PNI has shown that chronic or traumatic stress can disrupt both the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system. These disruptions contribute to serious health conditions, including cardiovascular diseases.
A key area of focus in PNI is the relationship between stress and coronary...
A key area of focus in PNI is the relationship between stress and coronary...
30
Introduction to Stress and Lifestyle
106
Stress is a multifaceted response to events perceived as challenging or threatening, highlighting physical, emotional, cognitive, and behavioral reactions. Physically, stress can lead to fatigue, sleep disruptions, and various health issues such as frequent colds, chest pains, and nausea. Emotionally, it can manifest as anxiety, depression, irritability, and anger triggered by both minor and major life events. Cognitively, it may result in difficulty in concentration, memory, and...
106
Hormones of the Adrenal Glands
2.4K
Adrenal hormones play a pivotal role in maintaining the body's electrolyte balance and orchestrating responses to stress, showcasing the intricate functions of the adrenal cortex and medulla.
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and...
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and...
2.4K
Peroxisomes
12.2K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
12.2K
