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在Taxus mairei中对BAHD酸转移酶家族的全基因组识别和表达模式分析

Donghuan Xu^1,2, Zhong Wang¹, Weibing Zhuang¹

¹Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China.

International journal of molecular sciences

|April 13, 2024

概括

研究人员在Taxus wallichiana var.中确定了123个BAHD乙烯转移酶. 马伊雷,其中52个可能参与税醇生物合成. 这项研究为TwBAHD基因家族及其在植物二次新陈代谢中的作用提供了基础的见解.

科学领域:

植物生物化学植物生物化学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

乙转移酶调节植物的二次新陈代谢.
在Taxus物种中,BAHD家族在很大程度上仍然没有特征.
了解这些酶对于税醇生物合成研究至关重要.

研究的目的:

在Taxus wallichiana var.中识别和描述BAHD乙烯转移酶基因家族. 马雷.马雷.马雷.马雷.马雷.马雷.马雷.马雷.马雷.马雷.马雷.
研究这些酶在税醇生物合成中的潜在作用.
为未来的功能研究提供一个全面的数据集.

主要方法:

对TwBAHD基因进行全基因组识别.
遗传学分析将TwBAHDs分类为分类.
对保存的图案和基因结构进行分析.
复制基因的Ka/Ks比计算.
对表达模式的RNA-seq分析 (MeJA诱导和组织特异).

主要成果:

确定了123个TwBAHD基因,并分为6个分类.
确定了52个成员的Taxus特定分支 (Clade VI),可能与taxol生物合成有关.
在类内观察到保存的基因 (包括YPLAGR) 和类似的基因结构.
协同复制的基因没有显著的功能差异化 (Ka/Ks < 1).
观察到不同的表达模式,在各种组织中高度表达的几个Taxus特定的TwBAHD.

结论:

这项研究提供了Taxus物种BAHD乙烯转移酶家族的首次全面分析.
塔克斯特有的TwBAHDs分支显示了潜在的参与taxol生物合成.
表达数据支持这些基因在Taxus二次新陈代谢中的功能意义.

关键词:

一辆出租车的马伊雷.TwBAHD 乙转移酶是一种表达方式表达方式表达模式整个基因组的识别.纳克索尔生物合成

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