相关实验视频

Updated: Jun 28, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

叶子衰老调节机制基于狐尾的比较转录组分析

Xiaoxi Zhen¹, Chao Liu¹, Yajun Guo¹

¹College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China.

International journal of molecular sciences

|April 13, 2024

概括

研究人员在狐尾小米中发现了调节叶子衰老的关键基因. 这项研究提高了对植物衰老的理解,并为改善作物产量和营养质量提供了遗传资源.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

叶子衰老对于作物产量和质量至关重要.
狐尾小米 (Setaria italica) 是一种有价值的C4模型作物,对衰老机制的研究有限.
识别与衰老相关的基因 (SAG) 和调节途径至关重要.

研究的目的:

在狐尾小米中调查暗感诱导衰老 (DIS) 期间的生理和转录组变化.
在轻度衰老 (LS) 和严重衰老 (SS) 品种之间比较衰老模式.
确定参与调节叶子衰老的新型SAG.

主要方法:

应用了一个黑暗诱导衰老 (DIS) 实验系统.
进行了生理和生化分析.
进行了比较转录组分析和权重基因同表达网络分析 (WGCNA).
使用RT-qPCR验证的结果.

主要成果:

LS品种显示延迟衰老;SS品种显示加速衰老.
观察到光合作用,叶绿素和脂质代谢基因表达的显著差异.
LS品种表现出多糖/结合,利用,防御和酸代谢基因的上调.
SS品种显示了与氧化还原平衡,碳水化合物/脂质代谢和激素信号传递相关的基因的改变表达.
确定了三种可能对DIS进行负面监管的SAG.

结论:

这项研究提供了对狐尾小米叶子衰老的分子调节的基础见解.
确定了潜在的遗传资源来操纵衰老以改善作物特征.
突出了与不同衰老率相关的不同基因表达模式.

关键词:

在黑暗中引起的衰老 (DIS)狐尾小米狐尾小米是一种小米.生理变化生理变化与衰老相关的基因 (SAGs)转录组分析转录组分析

更多相关视频

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Published on: August 18, 2023

In Situ Hybridization for the Precise Localization of Transcripts in Plants

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

相关概念视频

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...