橄果提取物含有低分子量,并具有抑制ACE的活性
Eduardo López-Huertas1, Jose Rubí-Villegas1, Lourdes Sánchez-Moreno2
1Group of Antioxidants and Free Radicals in Biotechnology, Food and Agriculture, Estación Experimental Zaidín (EEZ), Spanish National Research Council (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
International journal of molecular sciences
|April 13, 2024
概括
橄末提取物显示出显著的血管素转化酶 (ACE) 抑制活性. 这种农业副产品是生物活性,低分子量 (LMW) 的有希望的来源,用于健康应用.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 橄油,橄油生产的副产品,富含生物活性化合物.
- 低分子量 (LMW) 因其健康益处而越来越受认可.
- 抑制 ангиотензин转化酶 (ACE) 是管理高血压的关键目标.
研究的目的:
- 为了评估橄果汁水性提取物的ACE抑制活性.
- 为了识别和描述橄果汁中的生物活性LMW.
- 评估橄果汁作为营养和药理用途的来源的潜力.
主要方法:
- 橄果汁的水性提取和LMW分量的分离 (<3kDa).
- 使用纳米级液态染色学-Orbitrap与双重质谱学 (LC-MS/MS) 结合使用LMW分量的分析.
- 通过数据库搜索 (Peaks DB,BLAST) 和 de novo 测序来识别;通过 ACE 抑制活性预测 (BIOPEP).
主要成果:
- 每100克橄末的产量为100.2 ± 7.9毫克的LMW.
- LMW 分数表现出强大的 ACE 抑制活性 (IC50 = 3.57 ± 0.22 μg prot/mL).
- 确定了30种新的 (7-17种氨基酸,778-1354个Da),其中10种是通过de novo测序.
结论:
- 橄果具有显著的ACE抑制活性.
- 它是具有预测生物功能的生物活性LMW的宝贵来源.
- 对这些的体内作用进行进一步的研究对于营养和制药应用是有必要的.
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