源重编程碳和的新陈代谢,以促进安德罗格拉福利德生物合成在安德罗格拉菲斯·帕尼库拉塔 (Burm.f.) 需要幼苗
Shaofen Jian1,2,3, Si Wan1,2,3, Yang Lin1,2,3
1National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
International journal of molecular sciences
|April 13, 2024
概括
不同的源显著改变了Andrographis paniculata中的碳和代谢,影响了andrographolide等关键化合物的产生. 建议使用氨 (AN) 肥料,以提高安德罗格拉福利德的产量.
科学领域:
- 植物新陈代谢 植物新陈代谢
- 药用植物生物化学 药用植物生物化学
- 二次代谢物生产 二次代谢物生产
背景情况:
- (N) 代谢对于药用植物的二次代谢至关重要,但其在调节特特酸乳生物合成中的确切作用尚不清楚.
- 了解不同的N来源如何影响碳 (C) 和N代谢是优化像Andrographis paniculata这样的植物中宝贵化合物的生产的关键.
研究的目的:
- 研究不同源 (酸盐,,尿素,甘氨酸) 在Andrographis paniculata中重新编程C和N代谢的机制.
- 确定这些代谢转变对特特类乳,特别是andrographolide的合成和积累的影响.
主要方法:
- 安德罗格拉菲斯·帕尼库拉塔 (Andrographis paniculata) 种植时仅使用源:酸盐 (NN), (AN),尿素 (UN) 和甘氨酸 (GN).
- 进行了代谢物分析,酶活性测定和光合作用速率分析,以评估C和N代谢.
- 量化了二类乳,法纳西尔酸盐 (FPP),关键的糖分和TCA循环中间体以及植物激素的水平.
主要成果:
- 与酸盐 (NN) 相比, (AN),尿素 (UN) 和甘氨酸 (GN) 显著增加了主要安德罗格拉福利德成分和法尼西尔酸盐 (FPP) 的积累.
- 这些N来源改变了碳水化合物代谢,增加了光合作用和糖水平,同时降低了关键的糖解酶活动 (PFK,GAPDH,PEPC,PDH) 并增加了PEPCK和ME活动.
- AN,UN和GN抑制了N的同化,减少了TCA循环中间体,增强了阿尔金因和GABA代谢,增加了乙烯和酸 (SA) 水平,表明C/N平衡和激素信号的重新编程.
结论:
- 源对Andrographis paniculata的C和N代谢进行了深刻的重编程,将代谢流量从N同化转向增强的迪特尔类乳合成.
- 观察到的新陈代谢重编程,涉及改变碳水化合物分区和激素信号 (乙烯,SA),促进了andrographolide的积累.
- 建议使用 (AN) 肥料,以优化安德罗格拉福利德在安德罗格拉菲斯 (Andrographis paniculata) 种植中的产量.
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