水中的高NaCl度与斑马鱼的发育异常和改变基因表达有关
Denis A Seli1, Andrew Prendergast2, Yagmur Ergun1,3
1Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, New Haven, CT 06520, USA.
International journal of molecular sciences
|April 13, 2024
概括
由于盐污染造成的高盐度会损害斑马鱼的发育. 增加的化 (NaCl) 度会导致胚胎死亡,并影响基因表达,影响游泳膀的形成,并可能影响其他水生动物.
科学领域:
- 环境科学 环境科学
- 发展生物学 发展生物学
- 毒理学 毒理学 毒理学
背景情况:
- 盐是一种常见的生态系统污染物,影响水生生物.
- 盐度的变化会影响生物的生存和发育.
- 斑马鱼是研究发育毒性的模型生物.
研究的目的:
- 为了研究盐度增加对斑马鱼发育的影响.
- 为了确定特定盐 (NaCl,KCl,CaCl2,MgSO4) 对斑马鱼胚胎的影响.
- 为了确定盐度引起的发育异常背后的分子机制.
主要方法:
- 斑马鱼胚胎暴露于个体盐的度增加 (5×, 10×, 50×, 100×).
- 评估了生存,发育和游泳膀膨胀.
- 基因表达分析是使用RNA测序对胚胎细胞进行的.
主要成果:
- 化,化和硫酸没有引起显著异常.
- 高度的化 (50×,100×) 导致胚胎死亡.
- 较低的NaCl度 (5×,10×) 导致游泳膀缺陷和显著的差异性基因表达,包括Sonic Hedgehog通路基因的下调.
结论:
- 增加化度对斑马鱼的发育有害.
- NaCl诱导的发育异常与基因表达的改变有关,包括关键的发育途径.
- 研究结果表明,受到盐污染影响的水生生态系统存在潜在风险.
相关概念视频
Osmoregulation in Fishes
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
Responses to Salt Stress
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
Background and Environment Affect Phenotype
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...


