人类REV1对未受损和受损DNA的催化作用
Anastasia D Stolyarenko1,2, Anna A Novikova1,2, Evgeniy S Shilkin1,2
1National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
International journal of molecular sciences
|April 13, 2024
概括
人类REV1DNA聚合酶准确地合成与8-oxoG和O6-meG病变相反的DNA. 它还复制易发生错误的乙氨基甘醇,突出其在DNA修复和合成中的作用.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 遗传学是一种遗传学.
背景情况:
- REV1是一种支架蛋白,对DNA转化合成 (TLS) 至关重要.
- REV1具有Y家族DNA聚合酶活性,但其准确性和效率仍有争议.
- 了解REV1的功能是理解DNA修复机制的关键.
研究的目的:
- 描述全身人REV1.1的生物化学活性.
- 评估REV1在各种未损坏和损坏的DNA模板上的准确性和效率.
- 阐明REV1的基质偏好的结构基础.
主要方法:
- 来自Saccharomyces cerevisiae的全身人类REV1的表达和净化.
- 在不同DNA模板上的REV1的DNA聚合酶活性体外表征.
- 使用特定的DNA病变,包括8-oxoG,O6-meG,硫胺糖醇,青交叉链接和乙腺素.
主要成果:
- REV1表现出与8-oxoG和O6-meG相对准确的合成,效率中等.
- REV1以易发生错误的方式复制胺糖醇,并被GG青交叉链阻断.
- 生物化学数据支持模板A的Hoogsteen面对REV1活动的重要性.
结论:
- 人类REV1作为分布性DNA聚合酶,具有明显的病变特异性活动.
- REV1精确绕过某些病变的能力有助于基因组的稳定性.
- 该研究提供了关于REV1的机制及其在耐受DNA损伤中的作用的见解.
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