相关实验视频

Updated: Jun 28, 2025

Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein

Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein

Published on: October 9, 2011

在羊和山羊中,ORF病毒会导致促进瘤的炎症

Davide Pintus¹, Maria G Cancedda¹, Giantonella Puggioni¹

¹Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.

Veterinary pathology

|April 13, 2024

概括

奥尔夫病毒 (ORFV) 导致绵羊和山羊的非典型病变. ORFV触发了涉及表达EGFR和VEGFR2的巨细胞的炎症,通过vVEGF信号传递可能导致瘤状变化.

科学领域:

兽医病毒学兽医病毒学
在瘤学瘤学.
免疫学免疫学免疫学

背景情况:

奥尔夫病毒 (ORFV) 在绵羊和山羊中引起传染性湿疹 (ORF),呈现出各种皮肤和粘膜病变.
在一些ORF病例中观察到非典型的,类似瘤的变化,这表明了潜在的分子机制.
病毒和宿主因子在ORFV相关病原发生中的作用仍然不完全理解.

研究的目的:

调查导致ORFV感染非典型瘤样变化的分子因素.
识别参与ORF病变的病变发生的病毒和宿主分子.
探索ORFV诱导的分子变化的潜在致癌作用.

主要方法:

使用病毒培养和实时聚合酶链反应 (RT-PCR) 检测病毒.
损伤组织的组织病理学检查.
免疫组织化学,免疫光,以及病毒和宿主标记物的局部杂交 (vVEGF,VEGFR2,EGFR,CD163).
用于基因表达分析的定量RT-PCR (互白素-6,VEGFR2).

主要成果:

在受影响动物的病变组织和血液中检测到ORFV,令人惊的是,在受影响群体的健康山羊中检测到ORFV.
病变显示出伪乳头上皮的增殖和表达病毒VEGF (vVEGF) 和VEGFR2.2的新血管成分.
表皮生长因子受体 (EGFR) 在纤维血管成分,CD163+巨细胞和基本表皮表达.
CD163+巨与VEGF和VEGFR2相关,在受损组织中,白内素-6和VEGFR2基因过度表达.

结论:

ORFV感染会诱导一种由CD163+巨体表征的炎症反应.
这些巨细胞表达EGFR和VEGFR2,可能起到致癌作用.
这些发现表明ORFV的vVEGF信号传递与宿主炎症因子之间的协同作用促进了类似瘤的变化.

关键词:

在CD163+巨细胞中.皮肤上生长因子受体2 基因表达的基因表达方式一只山羊,一只山羊.帕拉波克斯病毒病毒.羊群羊群羊群的羊群是什么意思瘤是一个瘤.血管内皮生长因子血管内皮生长因子

更多相关视频

Intravitreal Injections in the Ovine Eye

Intravitreal Injections in the Ovine Eye

Published on: July 5, 2022

Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses

Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses

Published on: November 24, 2014

相关概念视频

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Mechanisms of Retrovirus-induced Cancers

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.