CircDiaph3通过miR-338-3p/SRSF1轴加剧H/R诱导的心肌细胞亡和炎症
1Department of Cardiovascular Medicine, PLA Southern Theater Command General Hospital, 11 Liuhua Road, Guangzhou, 510000, China.
Journal of bioenergetics and biomembranes
|April 13, 2024
概括
循环RNAcircDiaph3通过促进心肌细胞亡和炎症,加剧急性心肌梗塞. 准circDiaph3/miR-338-3p/SRSF1通路为心肌损伤提供了一个潜在的治疗策略.
科学领域:
- 心血管生物学 心血管生物学
- 分子医学是分子医学.
- 在RNA生物学,RNA生物学.
背景情况:
- 急性心肌梗塞 (AMI) 是全球死亡的主要原因之一.
- 缺氧/低氧化 (H/R) 诱导的心肌细胞损伤是AMI的主要驱动因素.
- 循环RNA与心血管疾病的发病有关.
研究的目的:
- 调查circDiaph3在心脏功能和H/R诱导的心肌细胞损伤中的作用.
- 阐明circDiaph3在心肌损伤中的功能背后的分子机制.
- 探索circDiaph3作为AMI的潜在治疗点.
主要方法:
- 建立一个AMI小鼠模型和H/R诱导的H9C2细胞模型.
- 使用RT-qPCR和生物信息学检测circDiaph3表达.
- 评估细胞活力,细胞亡,反应性氧物种和炎症性细胞因子.
- 对circDiaph3/miR-338-3p相互作用和miR-338-3p/SRSF1轴的研究.
主要成果:
- 在AMI患者,小鼠和H/R治疗细胞中,CircDiaph3表达升高.
- 在体内和体外,CircDiaph3静音降低了心肌细胞亡和炎症.
- 通过菌miR-338-3p,CircDiaph3促进了细胞亡和炎症,它准了SRSF1.1.
结论:
- CircDiaph3通过miR-338-3p/SRSF1轴加剧了H/R诱导的心肌细胞亡和炎症.
- 这种circDiaph3/miR-338-3p/SRSF1通路代表了心肌损伤的潜在治疗标.
- 这项研究为AMI的分子机制提供了新的见解.
相关概念视频
Myocarditis I: Introduction
Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
Rheumatic Heart Disease I: Introduction
Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...
Cardiomyopathy II: Dilated Cardiomyopathy
Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
Cardiomyopathy III: Hypertrophic Cardiomyopathy
Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
Cardiomyopathy IV: Restrictive Cardiomyopathy
Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
Inflammatory Bowel Disease III: Crohn's Disease
Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...


