从子污水中分离出来的vB_EcoM-P896大肠菌可以溶解肠道致病菌大肠杆菌和肠外致病菌大肠杆菌
Haiyan Zhang1,2,3, Xiazhu Su1,4, Xiangkuan Zheng1,4
1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
概括
一种新型细菌菌体,vB_EcoM-P896,有效地溶解了各种致病性大肠杆菌菌株,包括致病性大肠杆菌. 这种强大的T4类菌体在广泛的pH值和温度范围内表现出稳定性,表明对控制大肠杆菌感染的潜在临床应用.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 基因组学就是基因组学.
背景情况:
- 致病性大肠杆菌菌株对人类和动物健康构成重大威胁.
- 控制致病性大肠杆菌感染是具有挑战性的,因为不同的病变型,血清型和耐药性.
- 菌体为对抗细菌感染提供了一个有希望的替代方案.
研究的目的:
- 隔离和描述一种具有抗病原性大肠杆菌的性活性的新型细菌菌体.
- 评估分离的细菌菌体的稳定性和基因组特征.
- 确定细菌的遗传关系和潜在应用.
主要方法:
- 从子污水中分离了菌体vB_EcoM-P896.
- 对各种大肠杆菌病变型 (肠道和肠外) 的炎症活性测定.
- 稳定性测试 (温度,pH).
- 基因组测序 (Illumina HiSeq) 和 de novo 组装 (Spades) 进行.
- 形态分析 (TEM) 和遗传学分析 (MEGA6.0).
- 全基因组分析.
主要成果:
- 菌体vB_EcoM-P896对肠道和肠外病原体大肠杆菌,包括禽类病原体大肠杆菌表现出广泛的解毒活性.
- 该菌体在广泛的温度范围 (4-50°C) 和pH值 (3-11) 中表现出了显著的稳定性.
- 基因组测序揭示了170,656 bp dsDNA基因组与294个假定的编码区域. 形态和基因组分析将其归类为类似T4的髓病毒 (Caudovirales序列,Myoviridae家族).
- 遗传学分析证实vB_EcoM-P896是一种强大的T4类菌体.
结论:
- 菌体vB_EcoM-P896是一种强大而有效的抗各种致病性大肠杆菌菌株的药物.
- 它的稳定性和广泛的光学光谱表明,在临床环境中控制大肠杆菌感染的治疗应用有很大的潜力.
- 进一步研究其作为菌体治疗剂的应用是有必要的.
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