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相关概念视频

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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相关实验视频

Updated: Jun 28, 2025

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
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协议识别DNA结合蛋白识别核酸重复dsDNAs的协议.

Zhiyuan Huang1, Yixin Zhou1, Yang Liu1

  • 1Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

STAR protocols
|April 13, 2024
PubMed
概括

本研究介绍了一种识别与特定DNA重复相互作用的DNA结合蛋白的方法. 这有助于了解C9orf72肌缩侧面硬化症和前性痴呆症的遗传原因.

关键词:
细胞生物学 细胞生物学细胞培养培养的细胞培养.细胞分离/细胞分离.基于细胞的测试分子生物学分子生物学分子/化学探头 分子/化学探头蛋白质生物化学 蛋白质生物化学蛋白质的表达和净化过程

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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
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CD Spectroscopy to Study DNA-Protein Interactions
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相关实验视频

Last Updated: Jun 28, 2025

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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 神经科学是一个神经科学.

背景情况:

  • 结合DNA的蛋白质对于细胞功能至关重要,如生长调节和染色体组织.
  • 六核酸重复的扩大是C9orf72相关的肌缩侧面硬化症 (ALS) 和前性痴呆症 (FTD) 的主要遗传原因.

研究的目的:

  • 开发和介绍一项用于识别特定结合六核酸重复DNA序列的蛋白质的协议.
  • 通过表征相互作用的DNA结合蛋白来研究C9orf72-ALS和FTD背后的分子机制.

主要方法:

  • 利用基于SILAC (细胞培养中氨基酸的稳定同位素标记) 的方法来隔离和识别DNA结合蛋白.
  • 采用电泳运动转移试验 (EMSA) 来验证蛋白质-DNA相互作用.

主要成果:

  • 成功识别了能够特异识别双链六核酸重复DNA的蛋白质.
  • 该协议提供了一种强大的方法,用于发现参与重复扩张障碍的新型DNA结合蛋白.

结论:

  • 本方案允许发现与疾病相关的重复扩张相互作用的DNA结合蛋白.
  • 这项研究有助于了解C9orf72相关的神经退行性疾病的发病性.