相关实验视频

Updated: Jun 28, 2025

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

lincRNA00907通过准miRNA-942-5p/TAOK1p来促进NASH的进展

Gang Du¹, Zhaochen Jiang¹, Tong Xia¹

¹Organ Transplant Department, Qilu Hospital of Shangdong University, Jinan 250012, China.

|April 13, 2024

概括

长跨基因非编码RNA 00907 (linc00907) 通过调节脂质代谢和免疫力促进非酒精性脂肪肝炎 (NASH). 针对linc00907/miRNA-942-5p/TAOK1通路提供了潜在的NASH治疗策略.

科学领域:

分子生物学分子生物学
肝病学肝病学是一种肝病学.
生物化学生物化学

背景情况:

非酒精性脂肪肝炎 (NASH) 是一种进展性肝病,其特征是脂肪积累,炎症和细胞损伤.
纳什病原体背后的分子机制复杂且不完全理解,需要识别新的调节因素.

研究的目的:

研究长跨基因非编码RNA 00907 (linc00907) 在非酒精性脂肪肝炎 (NASH) 的发展和进展中的作用.
阐明linc00907影响脂质代谢和肝脏炎症反应的分子机制.

主要方法:

在使用qRT-PCR的NASH患者和健康对照者的肝脏组织中评估了linc00907的表达.
通过高脂肪饮食在小鼠模型中诱导了NASH,并使用棕酸/油酸建立了实验室细胞模型.
利用了Oil Red O染色,基因淘汰/过度表达和双露西法酶记者测试来分析linc00907对脂质积累,基因表达以及与miRNA-942-5p和TAOK1.1的相互作用的影响.

主要成果:

在NASH患者和小鼠模型中,Linc00907被显著上调.
Linc00907过度表达增加了细胞内脂质的积累,而敲击降低了它.
Linc00907充当miRNA-942-5p的ceRNA,对TAOK1的表达进行上调,促进脂质积累和减少亡,加剧NASH.

结论:

Linc00907通过隔离miRNA-942-5p促进NASH,导致TAOK1表达增加,脂质积累和炎症.
linc00907/miRNA-942-5p/TAOK1轴是NASH治疗的一个潜在的治疗点.

关键词:

纳什·纳什 (Nash Nash) 是一个名为纳什的城市.这就是TAOK1的意义.lincRNA009077 的相关信息微RNA-942-5p是一种小RNA.

更多相关视频

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease

Published on: March 24, 2023

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Published on: March 11, 2017

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...