微藻利用水产养殖料成分增强硫甲的共同代谢:共同代谢途径和酶机制
Peifang Wang1, Dingxin Li1, Min Sun1
1Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, People's Republic of China; College of Environment, Hohai University, Nanjing 210098, People's Republic of China.
Journal of hazardous materials
|April 13, 2024
概括
微藻可以降解水产养殖中的硫甲醇 (SMX) 抗生素. 料中的素显著增强了这种共同代谢生物降解,实现了高的SMX去除率,并为减少抗生素残留提供了可持续的解决方案.
科学领域:
- 环境微生物学 环境微生物学
- 水生毒理学 水生毒理学
- 生物修复是一种生物修复.
背景情况:
- 在水产养殖中使用抗生素会污染淡水环境.
- 人们对微藻在抗生素共代谢生物降解中的作用的理解有限.
- 水产养殖料成分可以作为微藻介导的共同代谢的基质.
研究的目的:
- 通过使用葡萄糖和素,研究Chlorella pyrenoidosa对硫甲醇 (SMX) 的共同代谢.
- 阐明微藻对SMX的共同代谢生物降解的机制.
- 确定参与SMX降解的关键酶和途径.
主要方法:
- 在葡萄糖和氨酸的存在下,Chlorella pyrenoidosa与SMX的共同培养.
- 使用质谱学量化SMX去除速率的量化.
- 密度函数理论 (DFT) 计算用于预测反应性位点.
- 酶活性测定和共同代谢物识别.
主要成果:
- 氨酸比葡萄糖更强烈地增强了 (1.5倍) SMX 同代谢.
- 在9mM-Lys系统中,最高的SMX去除率为68.77%±0.50%.
- 提出了五种共同代谢途径,包括硫原子键断裂,环分裂,氧化,乙化和谷氨基合.
- 鉴定出细胞染色体P450,血清酸酶和过氧化酶是关键酶.
结论:
- 微藻驱动的共同代谢,特别是由素增强,对水产养殖中的SMX生物降解是有效的.
- 了解催化机制为优化生物修复策略提供了基础.
- 这种方法提供了一种可持续的方法来减少水生环境中的硫胺抗生素残留物.
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