在批量反应器中通过Clostridium carboxidivorans优化CO发酵:介质组成的影响
F Lanzillo1, S Pisacane1, F Raganati1
1Department of Chemical, Materials and Production Engineering-Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli Italy.
Anaerobe
|April 13, 2024
概括
优化Clostridium carboxidivorans发酵的介质成分可以显著降低成本,同时保持溶剂生产. 关键因素包括酵母提取物度,纯氨酸作为减少剂,以及.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 微生物发酵 微生物发酵
- 代谢工程是代谢工程.
背景情况:
- 克洛斯特里 (Clostridium carboxidivorans) 是一种能够通过一氧化碳 (CO) 发酵产生溶剂的微生物.
- 优化发酵介质对于具有成本效益和高效的溶剂生产至关重要.
- 在不影响溶剂产量的情况下降低介质成本是工业应用中的一个关键挑战.
研究的目的:
- 为了研究介质成分对Clostridium carboxidivorans对CO发酵的影响.
- 为增强溶剂生产确定具有成本效益的介质组件.
- 确定是否需要特定的补充剂,如和.
主要方法:
- 酵母提取物 (YE) 度 (0-3 g/L) 的系统变化.
- 评估不同的还原剂:氨酸-HCl,纯氨酸,硫化和组合剂.
- 减少微量金属度和评估和的作用.
主要成果:
- 在优化条件下 (1 g/L YE,纯氨酸,75%的微量金属) 在150小时内产生1.39 g/L乙醇和0.27 g/L丁醇.
- 发现对于发酵来说是不必要的.
- 被认为对细胞生长和溶剂生产都至关重要.
结论:
- 中等优化对于通过Clostridium carboxidivorans的CO发酵生产成本效益高的溶剂至关重要.
- 降低的YE度,纯氨酸和较低的微量金属水平使发酵效率高.
- 是不可或缺的,而不需要最佳的发酵性能.
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