与糖相关的SnRK1a1介导的Opaque2酸化调节了玉米中的内种子填充
Tao Yang1, Yunqin Huang1, Longyu Liao1
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Molecular plant
|April 14, 2024
概括
糖糖通过调节Opaque2 (O2) 基因来刺激玉米核的发育. 蛋白激酶SnRK1a1通常会抑制O2,但糖糖会释放这种抑制,增强储存储量,并可能增加玉米产量.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 糖对于玉米内的填充至关重要,为储存提供碳,为合成提供信号.
- 关键转录因子,特别是通过关键转录因子,将糖水平与内的发育联系起来的分子调节仍然不清楚.
研究的目的:
- 阐明糖糖调节玉米内填充的分子机制.
- 确定Opaque2 (O2) 基因及其对糖的反应调节的作用.
主要方法:
- 研究了糖糖,Opaque2 (O2) 和蛋白质激酶SnRK1a1.1.之间的相互作用.
- 使用生物化学测试来确定O2上的酸化位点.
- 分析了SnRK1a1和O2变体的突变和过度表达系,以评估对种子特性的影响.
主要成果:
- 糖糖促进O2的表达,帮助储存储备的积累.
- SnRK1a1在血清41 (S41) 中酸化O2,降低其活性和稳定性;糖糖减轻了这种抑制.
- 改变SnRK1a1水平或O2酸化状态显著影响核的重量,大小和储存化合物积累 (粉和蛋白质).
结论:
- 发现了一种新的调节途径,其中糖糖通过S41的SNRK1a1酸化调节O2活性.
- 这种机制是协调碳供应与储存储备合成在玉米内填充期间的关键.
- 针对O2-SnRK1a1相互作用提出了一种改善玉米产量和储存能力的策略.
更多相关视频
06:11Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
Published on: September 22, 2023
2.9K
14:43Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Published on: July 23, 2014
13.4K
相关概念视频
Oligosaccharide Assembly
2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.8K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Role of Microtubules in Cell Wall Deposition
2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K
Glucose Transporters
22.7K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
22.7K
Phloem and Sugar Transport
37.3K
Like many living organisms, plants have tissues that specialize in specific plant functions. For example, shoots are well adapted to rapid growth, while roots are structured to acquire resources efficiently. However, sugar production is primarily restricted to the photosynthetic cells that reside in the leaves of angiosperm plants. Sugar and other resources are transported from photosynthetic tissues to other specialized tissues by a process called translocation.
37.3K
