在牛群中,SOX6 AU通过 cis-modulation 调节SOX6 AU来控制肌体发生
Xin Li, Shan-Shan Xing, Sheng-Bo Meng1
1College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.
Epigenetics
|April 14, 2024
概括
长非编码RNASOX6反感上游 (SOX6AU) 促进骨肌细胞的增殖,亡和分化在牛中. 这种lncRNA调节SOX6基因表达,影响肉牛的发育.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物科学 动物科学
背景情况:
- 长非编码RNAs (lncRNAs) 在调节骨肌肉发育中起着至关重要的作用.
- 许多 lncRNAs 在牛肌肉发育中的特定功能在很大程度上仍未被描述.
研究的目的:
- 为了研究新型 lncRNA SOX6 Antisense Upstream (SOX6 AU) 在牛骨肌肉发育中的作用.
- 阐明SOX6 AU调节肌肉细胞增殖,亡和分化的分子机制.
主要方法:
- 在牛中SOX6AU的鉴定和特征.
- 在西安牛肌肉不同发育阶段对SOX6 AU表达的分析.
- 在初级牛骨肌细胞中的体外淘汰和过度表达实验.
- RNA测序 (RNA-seq) 用于识别差异表达基因 (DEG).
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
- 西方斑点分析以评估蛋白质表达.
- 调查SOX6 AU和SOX6基因之间的监管关系.
主要成果:
- SOX6 AU被确定为SOX6基因的逆转录,并在发育过程中在牛肌肉中表现出差异性表达.
- SOX6 AU过度表达促进了牛初级骨肌细胞的增殖,亡和分化.
- RNA-seq分析显示,在SOX6 AU过度表达时,483个DEG被丰富在肌肉发育途径 (例如PI3K/AKT,MAPK) 中.
- 发现SOX6 AU可以抑制SOX6基因的mRNA和蛋白质表达.
- 击败SOX6还促进了牛骨肌细胞的增殖和亡.
- SOX6 AU通过SOX6.6的cis调节促进了骨肌肉细胞的增殖和亡.
结论:
- SOX6 AU是一种新型的lncRNA,在调节牛骨肌肉发育方面发挥着重要作用.
- SOX6 AU通过负调节SOX6基因,促进骨肌肉细胞的增殖,亡和分化.
- 这项研究揭示了牛肉肉类肌肉发育中的lncRNA的新型cis调节机制,为改善肉类生产提供了潜在的目标.
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