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来自致病性螺旋体Leptospira interrogans的LIC_12757编码了一个自我调节的ECF σ^E类型因子

Sabina Kędzierska-Mieszkowska¹, Barbara Kędzierska², Katarzyna Potrykus²

¹Department of General and Medical Biochemistry, Faculty of Biology, University of Gdańsk, Gdańsk 80-308, Poland.

Veterinary microbiology

|April 14, 2024

概括

这项研究描述了Leptospira interrogans中的extracytoplasmic功能sigma因子LIC_12757的特征. 我们展示了它作为调节基因表达和自身自我调节的转录因子的作用.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌病原体的产生

背景情况:

外细胞质功能 (ECF) σ因素对于细菌的适应性和毒性至关重要.
莱普托斯皮拉问答,一种致病原体,导致莱普托斯皮罗斯,编码多个不具特征的ECF σ因子.
LIC_12757是L. interrogans在高温上升调节的ECF σ因子,可能与毒性有关.

研究的目的:

来自L. interrogans.的ECF σ因子LIC_12757的生物化学特征.
阐明LIC_12757.7的转录调节作用.
为了研究LIC_12757.7.的自我调节.

主要方法:

使用pET系统进行基因克隆和基因表达.
电泳运动移动性转换试验 (EMSA).
在体外转录试验.

主要成果:

LIC_12757已成功克隆,表达和生物化学特征.
EMSA和体外转录证实LIC_12757作为转录因子的功能,将RNA聚合酶导向 σE型促进体.
发现LIC_12757在转录水平上是自我调节的.

结论:

LIC_12757是L. interrogans.中的一个功能转录因子.
这项研究提供了LIC_12757的第一个生化表征,这是了解其在Leptospira病原发生中的作用的关键一步.
LIC_12757的自我调节表明L. interrogans.中存在一个复杂的调节网络.

关键词:

感染感染感染莱普托斯皮拉 (Leptospira) 是一个类动物.莱普托斯皮罗斯症是什么?病原体是一种病原体.西格玛因子是指西格玛因子.转录转录转录转录

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