相关实验视频

Updated: Jun 28, 2025

Tracking Microbial Contamination in Retail Environments Using Fluorescent Powder - A Retail Delicatessen Environment Example

Tracking Microbial Contamination in Retail Environments Using Fluorescent Powder - A Retail Delicatessen Environment Example

Published on: March 5, 2014

开发一种基于罗达胺的光探针,用于ATP检测,用于肉类新鲜度评估的潜在应用

Yuanyuan Su¹, Minghui Gu¹, Cheng Li¹

¹Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

|April 14, 2024

概括

一个新的水溶性探针,罗达胺 - 乙烯胺 (RP),使得敏感和快速检测腺三酸盐 (ATP). 这个探测器显示了通过检测表面上的ATP来评估肉类新鲜性的潜力.

科学领域:

分析化学分析化学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

腺三酸盐 (ATP) 对于生理过程至关重要.
ATP是食品中微生物污染的关键指标.
开发敏感和快速检测ATP的方法对于食品安全和质量控制至关重要.

研究的目的:

开发一种新的,灵敏的,快速的,水溶性的光探针,用于检测腺三酸盐 (ATP).
为了调查探测器的检测机制.
评估探测器在实际应用中的潜力,例如评估肉类新鲜度.

主要方法:

罗达胺-乙烯胺 (RP) 探针的合成和特征使用福里埃变换红外线 (FTIR),核磁共振 (NMR) 和动态光散射 (DLS).
光谱法被用来监测探头对ATP的反应.
采用泽塔电位测量和分子动力学模拟来阐明检测机制.

主要成果:

合成的RP探头在与ATP结合时在580nm处表现出明显的光变化.
观察到光强度和ATP度在0-50μmol/L的范围内之间存在线性相关性.
对ATP的检测极限 (LOD) 确定为10.97 × 10−9 mol/L.
检测机制涉及多个正电荷的RP探针和ATP负电荷的三酸盐组之间的静电吸附.

结论:

开发的RP探头为ATP检测提供了灵敏,快速和水溶性的方法.
探测器的机制是基于静电相互作用,为小分子检测提供了洞察力.
在肉表面检测ATP的RP探针的成功应用表明其在评估食品新鲜性的实用性.

关键词:

这就是ATPATPATP ATP.光探测器探测器可以发光.多氨基酸多氨基酸.五乙烯胺的五乙烯胺.罗达胺 B 类型的罗达胺

更多相关视频

High-Resolution Fluoro-Respirometry of Equine Skeletal Muscle

High-Resolution Fluoro-Respirometry of Equine Skeletal Muscle

Published on: February 3, 2023

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...