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消除细胞外自诱导信号可以抑制金黄色葡萄球菌 (Staphylococcus aureus) 的定数感应系统

Ruki Inagaki¹, Ayaka Koshiba¹, Eri Nasuno¹

¹Graduate School of Regional Development and Creativity, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, 321-8585, Japan.

Biochemical and biophysical research communications

|April 14, 2024

概括

通过用纤维素膜捕获自身诱导 (AIPs) 来抑制金黄色葡萄球菌的毒性,有效抑制了毒素的产生. 这种策略使辅助基因调节 (agr) 定量感应 (qs) 系统保持不活跃,阻碍细菌感染.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

黄金葡萄球菌中的辅助基因调节 (agr) 定量感应 (QS) 系统对于毒性,毒素产生和宿主感染至关重要.
质量系统依赖于细胞间信号传导,通过在高细菌密度下释放的自诱导 (AIPs) 激活.

研究的目的:

为了评估通过捕获AIPs在Staphylococcus aureus培养过程中使用酸纤维素 (NC) 膜来抑制agr QS的有效性.
为了确定这种方法是否可以抑制依赖QS的毒性因子产生.

主要方法:

在培养基中浸泡NC膜的Staphylococcus aureus ATCC 12600培养. 在培养基中浸泡NC膜.
测量羊红细胞的血液溶解比率,使用培养超剂.
使用石英晶体微平衡分析来确认AIP对NC膜的吸附.
分析了agr QS基因 (agrA) 和α-hemolysin基因 (hla) 的表达水平.

主要成果:

浸泡NC膜显著降低了血液溶解比率,表明α-hemolysin生产受到了抑制.
石英晶体微平衡证实了AIP对NC膜的吸附.
与对照作物相比,添加NC膜保持了较低的agrA和hla表达水平.
细胞外AIP信号消除有效地使农业QS系统保持不活跃.

结论:

隔离细胞外信号,如AIPs,是一种有效的策略,可以抑制利用细胞间通信的细菌的基因表达.
这种方法表明,通过保持农业质量系统处于不活跃状态,可以控制金黄色葡萄球菌的毒性.

关键词:

配件基因调节器基因调节器自诱导的自诱导.血液溶解素 (Hemolysin) 是一种血液溶解剂.议员数量的灭.议员数量检测是指对议员数量进行检测.

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