鱼的皮肤粘膜表面成为抗生素耐药性基因的障碍,在阿布拉米辛暴露下
Wenpeng Li1, Xiaohan Zhang2, Xiaohan Hao1
1School of Marine Science and Technology, Tianjin University, Tianjin, 300072, China.
Environmental research
|April 14, 2024
概括
鱼皮粘膜表面 (SMS) 在水产养殖中暴露于抗生素时,作为抗生素耐药基因 (ARG) 的屏障. 在抗生素治疗后,SMS中富含的ARG会持续很长时间,这突显了它们的长期生态影响.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 水生毒理学 水生毒理学
背景情况:
- 抗生素耐药性基因 (ARG) 是一个日益严重的环境问题,特别是在抗生素普遍存在的水产养殖中.
- 鱼皮粘膜表面 (SMS) 是水生生物遇到环境污染物,包括抗生素的主要接口.
- 抗生素暴露对SMS的影响及其在ARG动态中的作用仍然不明.
研究的目的:
- 为了研究抗生素暴露对鱼皮粘膜表面 (SMS) 的影响.
- 在模拟水产养殖条件下确定ARG是否富含SMS.
- 探索SMS在ARG传输和持久性中的作用.
主要方法:
- 斑马鱼 (Danio rerio) 在实验室环境中暴露于抗生素阿普拉素 (APR) 和抗生素耐药细菌.
- 在APR暴露期间和之后,监测了SMS中的细菌丰度和多样性.
- 使用qPCR量化了SMS中的ARG丰度,并评估了非特异性免疫力和抗氧化蛋白活性.
主要成果:
- 尽管有阿普拉米辛和细菌暴露,但SMS有效地保持了细菌的丰富性和多样性的稳定性.
- 在停止APR暴露后的至少11天内,SMS中的ARG丰度仍然显著升高,表明持续的丰富.
- 非特异性免疫对于抵抗外部污染至关重要,而超氧化解突酶在抗氧化蛋白中表现出高活性.
结论:
- 鱼的皮肤粘膜表面 (SMS) 在暴露于阿布拉米辛下,作为抗生素耐药性基因 (ARG) 的持久屏障.
- 一旦被殖民,SMS中的ARG很难被消除,这表明在水生环境中存在长期的水库.
- 这项研究提供了关于ARG在水产养殖环境中的生态影响和传播动态的关键见解.
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