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Updated: Jun 28, 2025

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

丸功能障碍和暴露后"它的恢复效果"

Qi Zhang¹, YanLing Yang¹, Juan Liu¹

¹Department of Clinical Pharmacy, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, 445000, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|April 14, 2024

概括

暴露于会通过破坏生殖荷尔蒙和丸功能来损害男性生育能力. 康复是可能的,在停用后八周观察到生殖健康的完全恢复.

科学领域:

环境毒理学环境毒理学
生殖生物学生殖生物学
内分泌学在内分泌学.

背景情况:

工业化增加了对重金属的暴露,引发了人们对男性生育能力下降的担忧.
是一种普遍存在的环境污染物,与生殖毒性有关.
诱导的丸功能障碍的可逆性需要进一步调查.

研究的目的:

调查暴露停止后丸损伤和男性生育能力受损的可逆性.
阐明潜在的分子机制,包括激素轴调节和信号通路.
评估皮质在调节精子功能中的作用.

主要方法:

建立以受控暴露和随后撤销 (消除暴露) 的老鼠模型.
监测关键的生殖参数,包括生育能力,激素水平和丸功能指标.
对丸信号通路的分析,例如葡萄糖皮质体受体 (GR) /PI3K-AKT/AMPK.
在体外实验中使用皮质在精子上进行实验.

主要成果:

暴露降低了生育能力,抑制了下丘脑-垂体-丸轴,激活了下丘脑-垂体-上腺轴,并改变了丸GR/PI3K-AKT/AMPK信号传递.
丸细胞的增殖被抑制,暴露后亡增强.
停产四周后,生育率仍然下降,但指标显示出逆转的迹象.
停药8周后,所有观察到的指标都恢复到正常水平.
经证实,皮质可以通过GR/PI3K-AKT/AMPK通路来潜在地调节精子的增殖和亡.

结论:

暴露于会在雄性大鼠中引发显著的生殖毒性,影响生育能力和丸功能.
观察到的丸损伤和生育能力损害在停止暴露后是可逆的,在八周后完全恢复.
GR/PI3K-AKT/AMPK信号通路和皮质在调解的作用和随后的恢复过程中起着至关重要的作用.

关键词:

生育能力生育能力生育能力扩散和亡是它们的生长过程.恢复效应的恢复效应生殖健康生殖健康测试 (Testis) 是一个测试.

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