用二甲基硫氧化物调节细菌膜和细胞宏分子:依剂量依赖的研究提供了新的见解
Sinem Tunçer Çağlayan1, Rafig Gurbanov2
1Vocational School of Health Services, Department of Medical Services and Techniques, Bilecik Şeyh Edebali University, 11100 Bilecik, Turkey.
International journal of biological macromolecules
|April 14, 2024
概括
甲基二硫氧化物 (DMSO) 影响细菌生理学,甚至在低度下改变细胞膜和生物分子. 研究人员警告其在药物输送中的使用,因为这些非惰性细胞效应.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 甲基二硫氧化物 (DMSO) 在微生物学中被广泛用作溶剂.
- 了解DMSO对细菌生理学的影响对于其应用至关重要.
研究的目的:
- 研究DMSO与细菌细胞 (大肠杆菌) 之间的相互作用.
- 评估各种DMSO度对膜潜力,细胞表面电化学和生物分子组成的影响.
- 为了分析对DMSO治疗的反应中全基因组基因表达的变化.
主要方法:
- 用非增长抑制剂 (1.0%,2.5%) 和轻微抑制剂 (5.0%) 的DMSO治疗大肠杆菌.
- 测量细菌膜潜力和细胞表面电化学特征.
- 细胞生物分子组成和结构的分析.
- 全基因组基因表达特征分析.
主要成果:
- DMSO显著改变细菌膜潜力和细胞表面的电化学特性.
- DMSO会影响细胞生物分子的组成和结构.
- 基因表达分析揭示了细胞对DMSO暴露的反应.
结论:
- DMSO对细菌细胞具有非惰性作用,即使在低度下,也会影响细菌膜和大分子.
- 建议在使用DMSO用于药物输送时谨慎使用DMSO,因为它具有细胞效应.
- 研究结果提供了对DMSO与生物系统复杂相互作用的见解.
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