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鉴定了一种新型RNA转录TISPL,该转录被刺激ATF4的压力因素调高

Yutaro Wakabayashi¹, Aika Shimono², Yuki Terauchi²

¹Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan; Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), National Institute of Advanced Industrial Science and Technology (AIST), 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.

|April 14, 2024

概括

研究人员发现了一种新的转录,TISPL,作为细胞压力的生物标志物. 它的表达在各种压力条件下显著增加,由ATF4通路介导.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
发现生物标志物的发现.

背景情况:

细胞拥有检测和适应环境压力因素的机制.
细胞内膜网膜 (ER) 应激是一个关键的细胞状况.
之前的工作已经确定了对ER压力诱导因子敏感的记者细胞.

研究的目的:

识别和表征新型的对细胞应激有反应的转录.
为了研究参与压力诱导的基因表达的调节途径.
评估新发现的转录的潜力,作为细胞应激的生物标志物.

主要方法:

在HeLa细胞中利用了一种敏感的促进子陷向量系统.
使用Splinkerette PCR和cDNA末端的5'快速放大 (5'RACE) 进行转录识别.
在分析中进行,包括ChIP图谱,以确定转录因子结合位.
进行了ATF4淘汰实验,以阐明调节途径.

主要成果:

识别并指定了一个新的转录为TISPL (由LINC-PINT locus的压力因素诱导的转录).
观察到TISPL的显著上调 (大约. 在ER引发压力的1小时内 (10倍) (thapsigargin,tunicamycin).
发现TISPL表达在葡萄糖饥饿 (>10倍) 和化物暴露 (7倍) 下也增加.
已确认的TISPL上调取决于ATF4转录因子,这是综合应激反应的关键组成部分.

结论:

TISPL是一种新型的转录,通过ATF4通路通过各种细胞应激因素进行上调调节.
TISPL显示出作为一种敏感生物标志物的潜力,用于检测激活ATF4.4的压力条件.
敏感的陷载体系统对于发现新型应激反应生物标志物是有效的.

关键词:

在ATF4中使用ATF4.生物标志物生物标志物细胞内膜网膜应激压力综合应激反应综合应激反应长非编码RNA是什么意思

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