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Updated: May 5, 2026

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
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一种基于全血的功能性测定,以表征免疫球蛋白A效应器功能的特征

Alice Bacon1, Celia Cartagena García2, Karin A van Schie1

  • 1Rheumatology Department, Leiden University Medical Center (LUMC), Leiden, The Netherlands.

Autoimmunity
|April 15, 2024
PubMed
概括

与免疫球蛋白A1 (IgA1) 相比,免疫球蛋白A2 (IgA2) 子类IgA2m1和IgA2m2显示出更强的促炎性免疫细胞激活. 这一发现突出了IgA2的存在.

关键词:
在IgA效应器功能方面,IgA效应器功能.在IgA亚类中.这是全血,全血.流动细胞计量是流动细胞计量的方法.功能性检测试验的测试.这是一种炎症炎症炎症炎症.临床前研究研究.

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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