在巴格马提河,加德满都山谷的扩展谱β乳糖酶Escherichia Coli
Bindu Ghimire1, Muna Kumari Pokherel1, Megha Raj Banjara1
1Central Department of Microbiology, Tribhuvan University, Nepal.
Journal of Nepal Health Research Council
|April 15, 2024
概括
在巴格马提河中发现了产生多种药物耐药扩展谱β-乳糖酶的埃舍里希亚大肠杆菌,这表明潜在的循环和传播给人类.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性研究的研究.
- 公共卫生 公共卫生
背景情况:
- 在外围环境中的抗菌素耐药性 (AMR) 可以通过产生大肠杆菌的扩展谱β-乳糖酶 (ESBL) 来表示.
- 人类群体靠近水体,有助于AMR生物体进入人类生态系统.
- 在加德满都山谷的巴格马提河被调查存在ESBL产生的大肠杆菌.
研究的目的:
- 评估巴格马提河中产生β-乳糖酶的大肠杆菌的流行情况.
- 为了确定这些分离物的抗生素耐药性模式.
- 了解AMR在这个河流系统中的潜在公共卫生影响.
主要方法:
- 2020年进行了一项横截面研究,从巴格马提河沿线66个地点收集水样.
- 使用选择性介质 (在44.5°C时使用欧甲蓝) 隔离大肠杆菌,并通过培养和生物化学测试确定.
- 进行了抗生素敏感性测试 (Kirby Bauer磁盘扩散) 和多重聚合酶链反应,以检测β-乳酸酶基因.
主要成果:
- 在615个细菌分离物中,39% (n=241) 被确定为大肠杆菌.
- 在16.6% (40/241) 的大肠杆菌分离物中发现了产生扩展光谱β-乳糖酶的埃舍里希亚大肠杆菌,在40% (26/66) 的采样地点检测到.
- 所有大肠杆菌分离物都表现出多药性耐药性,耐药性率高 (>99%) 对青素,四环素和红素. 在产生ESBL和非产生ESBL的大肠杆菌 (p<0.05) 之间观察到对托和托的耐药性的显著差异.
结论:
- 巴格马提河中存在多药耐药ESBL产生的大肠杆菌 (Escherichia coli) 表明其在水生环境中循环.
- 这一发现突出了AMR生物体通过河流系统向人类传播的潜在途径.
- 该研究强调了监测河水质量的重要性,以监测AMR和保护公共健康.
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