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Updated: Jun 28, 2025

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

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双硫化物交叉连接的Tetra-PEG凝

Zhao Meng1,2, Lucas Löser3, Kay Saalwächter3

  • 1Institut des Matériaux and Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères, École Polytechnique Fédérale de Lausanne (EPFL), Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.

Macromolecules
|April 15, 2024
PubMed
概括

这项研究探讨了使用自我反应性聚乙烯糖醇 (PEG) 大分子制造二硫化物交联聚合物凝. 网络结构取决于宏分子度,在生物医学应用中提供了降解敏感水凝的潜力.

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相关概念视频

SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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