热逃生盒:用于研究热传感和热疼痛的成本效益评估范式
Jacquelyn R Dayton1, Jose Marquez1, Alejandra K Romo1
1University of California, Davis. Department of Physiology & Membrane Biology, 1275 Med Science Drive, Davis, CA 95616, United States.
Neurobiology of pain (Cambridge, Mass.)
|April 15, 2024
概括
研究人员开发了一种新的热逃生盒,以测量动物如何对温度做出决定. 这种工具有效地评估热感应和热疼痛,为温度感知及其障碍提供了新的见解.
科学领域:
- 神经科学是一个神经科学.
- 疼痛研究 疼痛研究
- 动物行为 动物行为
背景情况:
- 热感觉对于生存至关重要,但其损害会导致热疼痛综合征,如全发性痛和超痛性痛.
- 现有的动物热感应行为测试通常依赖于主观或反射反应.
- 需要客观,定量方法来研究热感应和热疼痛.
研究的目的:
- 开发和验证一种新型装置,即热逃生盒,用于评估小鼠的取决于温度的基于努力的决策.
- 通过使用新的测定方法,研究TRPM8和TRPV1通道在热传感中的作用.
- 评估热逃生盒在检测受伤或化疗引起的热疼痛方面的实用性.
主要方法:
- 开发了热逃生盒装置,将温度控制板块与基于选择的行为范式集成在一起.
- 通过视频录制分析量化小鼠逃离异常温度的延迟时间.
- 在野生型,Trpm8淘汰和Trpv1淘汰小鼠,以及在慢性收缩损伤和酸诱导的神经病变的模型中评估逃跑行为.
主要成果:
- 在极端温度 (5°C,18°C,52°C) 中,与中性温度 (30°C) 相比,小鼠的逃生延迟时间显著更长.
- 在Trpm8-/-和Trpv1-/-小鼠中,观察到的逃逸延迟的温度依赖差异被取消了.
- 慢性收缩损伤和氧化治疗都增加了在低温下逃逸的延迟时间,而 meloxicam 防止了氧化引起的感冒疼痛.
结论:
- 热逃生箱提供了强大的和客观的测量热感应和与温度相关的基于努力的决策.
- TRPM8和TRPV1通道是温度感知和逃生行为的关键调解者.
- 这种测定在检测神经病痛和化疗诱导的外围神经病变的动物模型中有效检测热疼痛,特别是感冒疼痛.
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