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Updated: Jun 28, 2025

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

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声学记者基因的定向进化使用高通量声学选

Robert C Hurt, Zhiyang Jin, Mohamed Soufi

    bioRxiv : the preprint server for biology
    |April 15, 2024
    PubMed
    概括

    研究人员设计了气囊 (GVs),以改善细胞的超声波成像. 定向进化增强了声学记者基因 (ARG),以在生物样本中实现更清晰的可视化.

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    Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming

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    Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

    Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

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    Reporter Genes02:11

    Reporter Genes

    Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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