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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K

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相关实验视频

Updated: Jun 28, 2025

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

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交叉相关性增加了基于扩散的超分辨率光学波动成像中的采样.

Jeanpun Antarasen, Benjamin Wellnitz, Stephanie N Kramer

    bioRxiv : the preprint server for biology
    |April 15, 2024
    PubMed
    概括
    此摘要是机器生成的。

    这项研究引入了扩散粒子的交叉相关性分析,增强了超分辨率成像. 这种新方法,光相关谱超分辨率光学波动成像 (fcsSOFI) 具有交叉相关性,同时揭示了纳米级的动态和结构.

    科学领域:

    • 光学成像和信号处理.
    • 纳米科学和生物物理学

    背景情况:

    • 超高分辨率成像技术经常与动态系统作斗争.
    • 现有的交叉关联方法仅限于静态或闪的发射器,而不是扩散颗粒.

    更多相关视频

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
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    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
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    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

    Published on: December 17, 2015

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    相关实验视频

    Last Updated: Jun 28, 2025

    From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
    15:10

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    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
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    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

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    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
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    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

    Published on: December 17, 2015

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    结论:

    • fcsSOFI与交叉相关性相结合,是纳米级成像的强大工具.
    • 这种方法使得在生物和软材料样本中,纳米级动态和结构的同时解析.