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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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相关实验视频

Updated: Jun 28, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

本佐HTag,一种使用分子进化开发的化自我标记蛋白质.

Bryan J Lampkin¹, Benjamin J Goldberg¹, Joshua A Kritzer¹

¹Department of Chemistry, Tufts University, Medford MA 02155.

bioRxiv : the preprint server for biology

|April 15, 2024

概括

研究人员开发了一种新的分子进化系统,以改善像HaloTag.这样的自我标记蛋白质. 这导致了BenzoHTag,使得更快,更明亮的活细胞标签使用西二醇染料用于化学生物学应用.

科学领域:

化学生物学化学生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

自标记蛋白质,如HaloTag7,通过遗传蛋白质融合促进合成分子的精确细胞定位.
HaloTag7的实用性受到许多基质的缓慢标记动力学限制,阻碍了活细胞测试应用.
快速标记率对于化学生物学中有效的活细胞测试至关重要.

结论:

分子进化方法显著提高了HaloTag与多种基质的性能.
与现有的HaloTag系统相比,BenzoHTag在活细胞测试中提供了更高的性能.
这项技术促进了化学生物学和药物发现的基于细胞的测试开发.

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017