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从70多岁的骨遗骸中获得的高度降解的DNA的Y-STR分析

Jiashuo Zhang¹, Xuebo Li^2,3, Liangliang Li^2,3

¹Research Center for Chinese Frontier Archaeology of Jilin University, Changchun, China.

Forensic sciences research

|April 15, 2024

概括

这项研究使用了骨遗骸中古DNA的Y-STR位点分析,以确认长期失踪人员的父系血统关系. 结果证实了家族联系,有助于通过罕见的Y-STR单元型鉴定.

科学领域:

法医人类学法医人类学
分子遗传学分子遗传学
古代DNA分析古代DNA分析

背景情况:

古代DNA提取技术的进步提高了从退化的骨遗骸中提取DNA概况.
Y-STR位点分析对于确定父系血统至关重要,特别是对于长期失踪人员.
识别70多岁的骨遗骸存在独特的法医挑战.

研究的目的:

确定失踪人员的骨遗骸及其所谓的亲属是否具有相同的Y-STR位置.
使用古代DNA分析建立父系关系.
评估Y-STR分析在识别长期失踪者的有用性.

主要方法:

从超过70岁的骨遗骸中提取古代DNA.
对22多个标记物进行Y-STR (Y染色体短并列重复) 位点的分析.
获得的Y-STR基因型与涉嫌亲属的个人资料和人口数据库 (Y-染色体STR单基因基因参考数据库) 的比较.

主要成果:

在所谓的亲属和失踪人员的共识资料中观察到相同的Y-STR基因型.
在汉族人口数据库中没有发现获得的Y-STR单元型,这表明稀有.
确认了失踪人7与家人1和失踪人18与家人2之间的父系关系.

结论:

Y-STR位点分析是一种高效的方法,用于在古代DNA样本中建立父系.
该研究成功地通过罕见的Y-STR单元型成功确定了失踪人员的家族联系.
这种方法在使用退化骨遗骸的过程中极大地帮助识别长期失踪人员.

关键词:

对Y-STR进行分析.法医遗传学法医遗传学高度退化的DNA.骨的残骸是一个骨残骸.

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